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Antioxidant peptides purified and identified from the oil palm (Elaeis guineensis Jacq.) kernel protein hydrolysate


Citation

Sui, Kiat Chang and Ismail, Amin and Yanagita, Teruyoshi and Mohd Esa, Norhaizan and Baharuldin, Mohamad Taufik Hidayat (2015) Antioxidant peptides purified and identified from the oil palm (Elaeis guineensis Jacq.) kernel protein hydrolysate. Journal of Functional Foods, 14. pp. 63-75. ISSN 1756-4646; ESSN: 2214-9414

Abstract / Synopsis

Protein extracted from oil palm kernel was hydrolyzed using pepsin-pancreatin (OPKH) and subsequently used to identify the potential antioxidative peptides. Then, the OPKH was purified sequentially by ultrafiltration, reversed-phase and semi-preparative HPLC. Antioxidant capacities (ACs) of the purified peptides from OPKH were evaluated using ferric reducing antioxidant power (FRAP), β-carotene-linoleate bleaching (BCB) assay and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging activity assay. Amino acid compositions of the purified peptide fractions were also determined. The amino acid sequence of the antioxidative peptide was identified by liquid chromatography-electrospray ionization/multi-stage mass spectrometry (LC-ESI-Q-TOF-MS/MS) using de novo sequencing. There were nine fractions purified and collected where F6 demonstrated the highest AC. Three antioxidative peptides, Val-Val-Gly-Gly-Asp-Gly-Asp-Val (VVGGDGDV), Val-Pro-Val-Thr-Ser-Thr (VPVTST) and Leu-Thr-Thr-Leu-Asp-Ser-Glu (LTTLDSE) were identified in fraction F6. These peptides did not show any similarity with other antioxidant peptides listed in BLAST database of NCBI. These peptides may be useful ingredients in food and pharmaceutical applications.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Halal Products Research Institute
DOI Number: https://doi.org/10.1016/j.jff.2015.01.011
Publisher: Elsevier BV
Keywords: Oil palm kernel protein hydrolysate (OPKH); Antioxidative peptide; Amino acid sequence; (LC-ESI-Q-TOF-MS/MS); Peptide purification; HPLC
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 09 Apr 2018 15:47
Last Modified: 09 Apr 2018 15:47
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.jff.2015.01.011
URI: http://psasir.upm.edu.my/id/eprint/43801
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