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Analysis of a1 and a2 globin genes among patients with hemoglobin Adana in Malaysia


Lee, T. Y. and Lai, M. I. and Ismail, P. and Ramachandran, V. and Tan, J. A. M. A. and Teh, L. K. and Othman, R. and Hussein, N. H. and George, E. (2016) Analysis of a1 and a2 globin genes among patients with hemoglobin Adana in Malaysia. Genetics and Molecular Research, 15 (2). pp. 1-8. ISSN 1676-5680


Hemoglobin (Hb) Adana [HBA2: c179G>A (or HBA1); p.Gly60Asp] is a non-deletional α-thalassemia variant found in Malaysia. An improvement in the molecular techniques in recent years has made identification of Hb Adana much easier. For this study, a total of 26 Hb Adana α-thalassemia intermedia and 10 Hb Adana trait blood samples were collected from patients. Common deletional and non-deletional α-thalassemia genotypes were determined using multiplex gap polymerase chain reaction (PCR) and multiplex ARMS PCR techniques. Identification of the Hb Adana location on the α-globin gene was carried out using genomic sequencing and the location of the mutation was confirmed via restriction fragment length polymorphism-PCR. Among the 36 samples, 24 (66.7%) had the -α3.7/αCd59α mutation, while the -α3.7/αCd59α mutation accounted for 2 samples (5.6%) and the remaining 10 (27.8%) samples were α/αCd59α. All 36 samples were found to have the Hb Adana mutation on the a2-globin gene. The position of the α-globin gene mutation found in our cases was similar to that reported in Indonesia (16%) but not to that in Turkey (0.6%). Our results showed that the Hb Adana mutation was preferentially present in the a2-globin genes in Malays compared to the other ethnicities in Malaysia. Thus, the Malays might have similar ancestry based on the similarities in the Hb Adana position.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Modern Language and Communication
Malaysian Research Institute on Ageing
DOI Number: https://doi.org/10.4238/gmr.15027400
Publisher: Fundacao de Pesquisas Cientificas de Ribeirao Preto
Keywords: Hb Adana; α-thalassemia; α-globin genes; RFLP-PCR; Genotyping
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 09 Apr 2018 06:19
Last Modified: 09 Apr 2018 06:19
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.4238/gmr.15027400
URI: http://psasir.upm.edu.my/id/eprint/43750
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