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α-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPARgamma, GLUT4, and leptin expressions


Taher, Muhammad and Mohamed Amiroudine, Mohamed Zaffar Ali and Tengku Zakaria, Tengku Muhamad Faris Syafiq and Deny Susanti, and Jauhari Arief Ichwan, Solachuddin and Kaderi, Mohd Arifin and Ahmed, Qamar Uddin and Zakaria, Zainul Amiruddin (2015) α-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPARgamma, GLUT4, and leptin expressions. Evidence-Based Complementary and Alternative Medicine, 2015. art. no. 74023. pp. 1-9. ISSN 1741-427X; ESSN: 1741-4288


Obesity has been often associated with the occurrence of cardiovascular diseases, type 2 diabetes, and cancer. The development of obesity is also accompanied by significant differentiation of preadipocytes into adipocytes. In this study, we investigated the activity of α-mangostin, a major xanthone component isolated from the stem bark of G. malaccensis, on glucose uptake and adipocyte differentiation of 3T3-L1 cells focusing on PPARγ, GLUT4, and leptin expressions. α-Mangostin was found to inhibit cytoplasmic lipid accumulation and adipogenic differentiation. Cells treated with 50 μM of α-mangostin reduced intracellular fat accumulation dose-dependently up to 44.4% relative to MDI-treated cells. Analyses of 2-deoxy-D-[3H] glucose uptake activity showed that α-mangostin significantly improved the glucose uptake ( P < 0.05 ) with highest activity found at 25 μM. In addition, α-mangostin increased the amount of free fatty acids (FFA) released. The highest glycerol release level was observed at 50 μM of α-mangostin. qRT-PCR analysis showed reduced lipid accumulation via inhibition of PPARγ gene expression. Induction of glucose uptake and free fatty acid release by α-mangostin were accompanied by increasing mRNA expression of GLUT4 and leptin. These evidences propose that α-mangostin might be possible candidate for the effective management of obesity in future.

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Official URL or Download Paper: http://www.hindawi.com/journals/ecam/2015/740238/

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1155/2015/740238
Publisher: Hindawi Publishing Corporation
Keywords: Obesity; α-mangostin
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 08 Aug 2016 09:24
Last Modified: 08 Aug 2016 09:24
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2015/740238
URI: http://psasir.upm.edu.my/id/eprint/43690
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