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Metabolic alteration in obese diabetes rats upon treatment with Centella asiatica extract


Maulidiani, and Abas, Faridah and Khatib, Alfi and Perumal, V. and Suppaiah, Velan and Ismail, A. and Hamid, Muhajir and Shaari, Khozirah and Lajis, Md. Nordin (2016) Metabolic alteration in obese diabetes rats upon treatment with Centella asiatica extract. Journal of Ethnopharmacology, 180. pp. 60-69. ISSN 0378-8741; ESSN: 1872-7573


Ethnopharmacological relevance: ‘Pegaga’ is a traditional Malay remedy for a wide range of complaints. Among the 'pegaga’, Centella asiatica has been used as a remedy for diabetes mellitus. Thus, we decided to validate this claim by evaluating the in vivo antidiabetic property of C. asiatica (CA) on T2DM rat model using the holistic 1H NMR-based metabolomics approach. Method: In this study, an obese diabetic (mimic of T2DM condition) animal model was developed using Sprague–Dawley rats fed with a high-fat diet and induced into diabetic condition by the treatment of a low dose of streptozotocin (STZ). The effect of C. asiatica extract on the experimental animals was followed based on the changes observed in the urinary and serum metabolites, measured by 1H NMR of urine and blood samples collected over the test period. Results: A long-term treatment of obese diabetic rats with CA extract could reverse the glucose and lipid levels, as well as the tricarboxylic acid cycle and amino acid metabolic disorders, back towards normal states. Biochemical analysis also showed an increase of insulin production in diabetic rats upon treatment of CA extract. Conclusion: This study has provided evidence that clearly supported the traditional use of CA as a remedy for diabetes. NMR-based metabolomics was successfully applied to show that CA produced both anti-hyperglycemic and anti-hyperlipidemic effects on a rat model. In addition to increasing the insulin secretion, the CA extract also ameliorates the metabolic pathways affected in the induced diabectic rats. This study further revealed the potential usage of CA extract in managing diabetes mellitus and the results of this work may contribute towards the further understanding of the underlying molecular mechanism of this herbal remedy.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Food Science and Technology
Faculty of Medicine and Health Science
Institute of Bioscience
DOI Number: https://doi.org/10.1016/j.jep.2016.01.001
Publisher: Elsevier
Keywords: Centella asiatica; NMR-based metabolomics; Obese-diabetic rats; Urine metabolites; Serum metabolites
Depositing User: Nabilah Mustapa
Date Deposited: 19 May 2016 03:56
Last Modified: 19 May 2016 03:56
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.jep.2016.01.001
URI: http://psasir.upm.edu.my/id/eprint/43424
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