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Protective effects of the extracts of Barringtonia racemosa shoots against oxidative damage in HepG2 cells


Citation

Kong, Kin Weng and Mat Junit, Sarni and Aminudin, Norhaniza and Hassan, Fouad Abdulrahman and Ismail, Amin and Abdul Aziz, Azlina (2016) Protective effects of the extracts of Barringtonia racemosa shoots against oxidative damage in HepG2 cells. PeerJ, 4. art. no. 1628. pp. 1-20. ISSN 2167-8359

Abstract

Barringtonia racemosa is a tropical plant with medicinal values. In this study, the ability of the water extracts of the leaf (BLE) and stem (BSE) from the shoots to protect HepG2 cells against oxidative damage was studied. Five major polyphenolic compounds consisting of gallic acid, ellagic acid, protocatechuic acid, quercetin and kaempferol were identified using HPLC-DAD and ESI-MS. Cell viability assay revealed that BLE and BSE were non-cytotoxic (cell viabilities >80%) at concentration less than 250 µg/ml and 500 µg/ml, respectively. BLE and BSE improved cellular antioxidant status measured by FRAP assay and protected HepG2 cells against H2O2-induced cytotoxicity. The extracts also inhibited lipid peroxidation in HepG2 cells as well as the production of reactive oxygen species. BLE and BSE could also suppress the activities of superoxide dismutase and catalase during oxidative stress. The shoots of B. racemosa can be an alternative bioactive ingredient in the prevention of oxidative damage.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.7717/peerj.1628
Publisher: PeerJ
Keywords: Antioxidant enzymes; Barringtonia racemosa; HPLC-ESI-MS; Lipid peroxidation; Oxidative stress; Polyphenols
Depositing User: Nabilah Mustapa
Date Deposited: 12 Apr 2016 07:55
Last Modified: 12 Apr 2016 07:55
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.7717/peerj.1628
URI: http://psasir.upm.edu.my/id/eprint/42279
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