UPM Institutional Repository

Isolation and characterization of Lactobacillus strains as potential probiotics for chickens


Shokryazdan, Parisa and Ramasamy, Kalavathy and Sieo, Chin Chin and Mohamed Alitheen, Noorjahan Banu and Liang, Juan Boo and Jahromi, Mohammad Faseleh and Ho, Yin Wan (2014) Isolation and characterization of Lactobacillus strains as potential probiotics for chickens. Pertanika Journal of Tropical Agricultural Science, 37 (1). pp. 141-157. ISSN 1511-3701; ESSN: 2231-8542


The ban on the use of antibiotics as growth promoters for poultry production in many countries has led to increasing interest to use probiotics as an alternative. In the present study, some Lactobacillus strains were isolated from chicken intestines, identified and assessed (in vitro) for their ability to survive and colonize the gastrointestinal tract (GIT), with a view to select suitable strains as potential probiotic candidates for chickens. Out of 42 isolated strains, three isolates, identified as Lactobacillus strains based on initial identification and tolerant to acid and bile based on preliminary screening using turbidity (optical density) as a measurement of growth, were selected for detailed identification and further in vitro assays. The three isolates were identified to species level using carbohydrate fermentation profile analysis and 16S rRNA gene sequencing. Results showed that all three strains belonged to Lactobacillus salivarius. The three L. salivarius strains were then assessed for their ability to tolerate the stress conditions in the GIT and capacity to adhere to the intestinal epithelial cells using in vitro assays of acid, bile and pancreatic enzyme tolerance measured by viable colony counts, and adhesion assay using Caco-2 cell line. The results showed that all three L. salivarius strains exhibited good tolerance to acid, bile and pancreatic enzymes and a strong ability to adhere to intestinal epithelial cells. Thus, they would be able to survive the stress conditions of GIT, as well as to attach and colonize the GIT, and could be considered as good potential candidates for probiotics of chickens.

Download File

Isolation and characterization of lactobacillus strains as potential probiotics for chickens.pdf

Download (482kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
Institute of Tropical Agriculture
Publisher: Universiti Putra Malaysia Press
Keywords: Chicken probiotic; Lactobacillus strains; Lactobacillus salivarius; Probiotic Lactobacillus
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 10 Feb 2016 04:59
Last Modified: 10 Feb 2016 04:59
URI: http://psasir.upm.edu.my/id/eprint/41782
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item