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Antibody responses in chickens experimentally infected with low and high passaged chicken anemia virus isolates


Citation

Chowdhury, Shah Md. Ziqrul Haq and Omar, Abdul Rahman and Ideris, Aini and Darus, Azizah and Kono, Yuji (2005) Antibody responses in chickens experimentally infected with low and high passaged chicken anemia virus isolates. Jurnal Veterinar Malaysia, 17 (1). pp. 13-17. ISSN 9128-2506

Abstract / Synopsis

It has been shown that chicken anemia virus (CAV) which had undergone 60 and 123 passages in cell cultures (SMSC-1IP60, SMSC-I/P123, 3-1IP60 and 3-l/P123) were less pathogenic compared to low passaged CAY (SMSC- 1 and 3-1) isolates. In this study, the ability of the isolates to induce antibody responses was studied using enzymelinked immunosorbent assay (ELISA). All the isolates regardless of the number of cell culture passages that they had undergone elicited CAY antibody responses both at 16 and 30 days post inoculation. A CAY isolate, BL-5 that was not passaged in cell culture elicited higher antibody response than the cell culture passaged isolates. However, the differences in the average ELISA titres and the percentage of positive sera between the isolates were not statistically significant (P>0.05). The study showed that CAY isolates which had undergone repeated passages in cell culture are still immunogenic


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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Publisher: Veterinary Association Malaysia
Keywords: Chicken anemia virus; Antibody response; Enzyme linked immunosorbent assay
Depositing User: Azhar Abdul Rahman
Date Deposited: 22 Dec 2015 08:37
Last Modified: 22 Dec 2015 08:37
URI: http://psasir.upm.edu.my/id/eprint/41534
Statistic Details: View Download Statistic

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