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Topical effects of haruan (Channa striatus bloch)-based cream on inflammation in rats and mice


Citation

Abedi, Sadegh (2012) Topical effects of haruan (Channa striatus bloch)-based cream on inflammation in rats and mice. Masters thesis, Universiti Putra Malaysia.

Abstract

Natural treatments with little side effects can be very good substitutes as chemical therapeutics. Haruan (Channa striatus, Channidae) is a stripped snakehead, tropical, freshwater, carnivorous, and air breathing fish species, indigenous to Malaysia. The main objective of the current study was to study the topical anti-inflammatory properties of haruan (Channa striatus) based cream in acute and chronic inflammation induced animals. Changes in ear weight, thickness, and myeloperoxidase level in croton oil-induced mice ear edema were measured as acute anti-inflammatory activities, and changes in right hind paw volume in complete Freund’s adjuvant-induced arthritis was measured as chronic anti-inflammatory activity of haruan based cream. Hydrocortisone 1 % cream and diclofenac 1% cream were applied as reference drug in acute and chronic phases; respectively. At 24 hours, the treatment dosages of haruan based cream reduced ear edema by 68 %,78 %, and 98 %, respectively, whereas hydrocortisone is 92 %. Data also showed that haruan based cream of 10 %, at 24 hours was significantly (p < 0.05) more effective than 5% in inhibiting ear edema. Mean mass of ear punch biopsies in treatment groups 1, 2, and 3, and hydrocortisone were 1.63 ± 0.08, 1.57 ± 0.06, 1.32 ± 0.01, and 1.50 ± 0.05 mg (mean ± SEM), respectively, compared to 2.19 ± 0.17 mg (mean ± SEM) in vehicle group. Mean MPO concentration of biopsies taken from ears treated with hydrocortisone cream 1% and haruan based cream at 1%, 5%,and 10% were 3.20 ± 0.080, 3.74 ± 0.110, 3.44 ± 0.060, and 2.72 ± 0.025 ng/ml (mean ± SEM), respectively. Average of paw volume changes at 21 days after inducing arthritis, in animals treated with diclofenac sodium cream 1%, and haruan based cream 1%, 5%, and 10% were 0.390 ± 0.122, 0.465 ± 0.080, 0.535 ± 0.190,and 0.043 ± 0.016 ml (mean ± SEM), respectively. In comparison with vehicle group (2.76 ml), all three treatment groups and reference group had significantly reduced paw edema (p < 0.05). As conclusion, it is suggested that the topical antiinflammatory effects of haruan based cream is contributed by its bioactive substances content especially fatty acids and amino acids.


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Additional Metadata

Item Type: Thesis (Masters)
Subject: Anti-Inflammatory Agents, Non-Steroidal
Subject: Anti-infective agents, Local
Subject: Animal Testing Alternatives
Call Number: FPSK(m) 2012 38
Chairman Supervisor: Professor Abdul Manan Mat Jais, PhD
Divisions: Faculty of Medicine and Health Science
Depositing User: Haridan Mohd Jais
Date Deposited: 26 Nov 2015 04:03
Last Modified: 26 Nov 2015 04:03
URI: http://psasir.upm.edu.my/id/eprint/41493
Statistic Details: View Download Statistic

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