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Transcriptomic analysis on susceptibility of different inbred chicken lines towards very virulent infectious bursal disease virus infection


Citation

Mohd Isa, Nor Farah and Bejo, Mohd Hair and Mat Isa, Nurulfiza and Ideris, Aini and Nair, Venugopal and Omar, Abdul Rahman (2013) Transcriptomic analysis on susceptibility of different inbred chicken lines towards very virulent infectious bursal disease virus infection. In: World’s Poultry Science Association (Malaysia Branch) and World Veterinary Poultry Association (Malaysia Branch) Scientific Conference 2013, 30 Nov.-1 Dec. 2013, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (pp. 126-127).

Abstract

Previous studies have identified differential expression of immune-mediated genes related to inflammatory response in chickens with different genetic susceptibility to IBDV infections. However, the mechanisms of genetic resistance against IBD are not known. RNA sequencing through NGS technologies provide an excellent platform to study differentially expressed genes of known or unknown functions to better define mechanisms of host resistance. Therefore, this study aimed at investigating susceptibility of different inbred chicken lines toward very virulent IBDV through transcriptomic analysis. This analysis allows for quantification and comparison of gene expression. Bioinformatics analysis of this data will allow function annotation of differentially expressed genes, indicating possible roles in the response to infection. Gene of interest, virus load detection, indel, SNP and CNV between different lines and breeds will be validated using qPCR. This study is expected to provide information that able to decipher the genetic resistance of chickens towards IBDV infection.


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Additional Metadata

Item Type: Conference or Workshop Item (Poster)
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Veterinary Medicine
Institute of Bioscience
Publisher: Faculty of Veterinary Medicine, Universiti Putra Malaysia
Keywords: Infectious bursal disease; Chicken; RNA; Genes; Vaccines
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 16 Nov 2015 09:07
Last Modified: 28 Jan 2016 02:04
URI: http://psasir.upm.edu.my/id/eprint/41414
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