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S-phase fraction and pro-inflammatory cytokine expressions in acute lymphoblastic leukaemia


Citation

Abdullah, Maha and Cheong, Soon Keng and Leong , Chooi Fun and Seow, Heng Fong (2005) S-phase fraction and pro-inflammatory cytokine expressions in acute lymphoblastic leukaemia. Malaysian Journal of Medicine and Health Sciences, 1 (1). pp. 41-51. ISSN 1675-8544

Abstract / Synopsis

Despite much progress in treatment strategies, long term survival of adult ALL is still inferior to that in children. The underlying mechanisms for these differences are largely unknown. Intensification of contemporary therapy has also resulted in many children being over-treated. The action of chemotherapeutic drugs used in the treatment al ALL includes cell cycle dependent agents which are effective on cells that are proliferating. Cell proliferation in haemopoietic cells in controlled by cytikines. Thus, we proposed to study the cell cycle profile of ALL cases and also expression of cytokines to determine their role in affecting treatment outcome in the different age groups. Methods: We determined the S-phase fraction from the cell cycle profile by flowcytometry and tested expressions of cytokine IL-1β, IL-18, IFN-μ, TNF-α and GM-CSF using RT-PCR in de novo ALL cases. Results: We found a significantly higher S-phase fraction in smaples from children 2-10 years old compared to the older age group (>10 years old) (p=0.001). GM-CSF was found to be expressed in significantly lower percentage of children compared to adults (p=0.008). Conclusion: Our results implied that GM-CSF may have induces cell cycle arrest in adult ALL resulting in a lower percentage of S-phase fractions. This may contribute to the poorer prognosis in adults ALL because non-cycling blast are less sensitive to some chemotherapeutic drugs.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Publisher: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
Keywords: ALL; S-phase fraction; GM-CSF; Age
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 03 Dec 2015 09:27
Last Modified: 03 Dec 2015 09:27
URI: http://psasir.upm.edu.my/id/eprint/41121
Statistic Details: View Download Statistic

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