Simple Search:

Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species


Citation

Ahmad, Rohaya and Ali, Abdul Manaf and Ahmad Israf, Daud and Ismail, Nor Hadiani and Shaari, Khozirah and Lajis, Nordin (2005) Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species. Life Sciences, 76 (17). pp. 1953-1964. ISSN 0024-3205; ESSN: 1879-0631

Abstract / Synopsis

The antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of seven Hedyotisspecies were investigated. The antioxidant activity was evaluated by the ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods while the radical scavenging activity was measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The anti-inflammatory activity related to NO inhibition of the plant extracts was measured by the Griess assay while cytotoxicity were measured by the MTT assay against CEM-SS cell line. The antibacterial bioassay (against 4 bacteria, i.e. Bacillus subtilis B28 (mutant), Bacillus subtilis B29 (wild-type), Pseudomonas aeruginosa UI 60690 and methicillin resistant Staphylococcus aureus, (MRSA) was also carried out using the disc-diffusion method. All tested extracts exhibited very strong antioxidant properties when compared to Vitamin E (α-tocopherol) with percent inhibition of 89–98% in the FTC and 60–95% in the TBA assays. In the DPPH method, H. herbacea exhibited the strongest radical scavenging activity with an IC50 value of 32 μg/ml. The results from the Griess assay showed that the tested extracts are weak inhibitors of NO synthase. However, all tested extracts exhibited moderate cytotoxic properties against CEM-SS cell line giving CD50 values in the range of 21–41 μg/ml. In the antibacterial bioassay, the stems and the roots of H. capitellata showed moderate activity against the 4 tested bacteria while the leaves showed moderate activity towards B. subtilis B28, MRSA and P. aeruginosa only. The roots of H. dichotoma showed strong antibacterial activity against all 4 bacteria. All other extracts did not exhibit any antibacterial activity.


Download File

[img]
Preview
PDF (Abstract)
Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species.pdf

Download (51kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Biotechnology
Faculty of Medicine and Health Science
Institute of Bioscience
DOI Number: https://doi.org/10.1016/j.lfs.2004.08.039
Publisher: Elsevier
Keywords: Antioxidant; Radical scavenging; Anti-inflammatory; NO inhibition; Cytotoxic; Antibacterial; Hedyotis
Depositing User: Nabilah Mustapa
Date Deposited: 17 Sep 2015 09:34
Last Modified: 17 Sep 2015 09:34
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.lfs.2004.08.039
URI: http://psasir.upm.edu.my/id/eprint/40322
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item