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Loss of mitochondrial transmembrane potential and caspase-9 activation during apoptosis induced by the novel styryl-lactone goniothalamin in HL-60 leukemia cells


Citation

Inayat Hussain, Salmaan Hussain and Osman, Md. Anuar and Din, Laily and Ali, Abdul Manaf and Ross, David (2003) Loss of mitochondrial transmembrane potential and caspase-9 activation during apoptosis induced by the novel styryl-lactone goniothalamin in HL-60 leukemia cells. Toxicology in Vitro, 17 (4). pp. 433-439. ISSN 0887-2333; ESSN: 1879-3177

Abstract

Styryl-lactones such as goniothalamin represent a new class of compounds with potential anti-cancer properties. In this study, we investigated the mechanisms of goniothalamin (GTN), a plant styryl-lactone induced apoptosis in human promyelocytic leukemia HL-60 cells. This plant extract resulted in apoptosis in HL-60 cells as assessed by the externalisation of phosphatidylserine. Using the mitochondrial membrane dye (DIOC6) in conjunction with flow cytometry, we found that GTN treated HL-60 cells demonstrated a loss of mitochondrial transmembrane potential (Δψm). Further immunoblotting on these cells showed activation of initiator caspase-9 and the executioner caspases-3 and -7. Pretreatment with the pharmacological caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp fluoromethyl ketone (Z-VAD.FMK) abrogated apoptosis as assessed by all of the apoptotic features in this study. In summary, our results demonstrate that goniothalamin-induced apoptosis occurs via the mitochondrial pathway in a caspase dependent manner.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Biotechnology
DOI Number: https://doi.org/10.1016/S0887-2333(03)00051-1
Publisher: Elsevier
Keywords: Apoptosis; Caspase activation; Goniothalamin; Mitochondrial transmembrane potential; Styryl-lactone
Depositing User: Nabilah Mustapa
Date Deposited: 17 Sep 2015 01:00
Last Modified: 24 Jul 2024 06:28
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/S0887-2333(03)00051-1
URI: http://psasir.upm.edu.my/id/eprint/40315
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