UPM Institutional Repository

S-nitrosylation of NADPH oxidase regulates cell death in plant immunity


Yun, Byung Wook and Feechan, Angela and Yin, Minghui and Saidi, Noor Baity and Le Bihan, Thierry and Yu, Manda and Moore, John W. and Kang, Jeong Gu and Kwon, Eunjung and Spoel, Steven H. and Pallas, Jacqueline A. and Loake, Gary J. (2011) S-nitrosylation of NADPH oxidase regulates cell death in plant immunity. Nature, 478 (7368). pp. 264-268. ISSN 0028-0836; ESSN: 1476-4687


Changes in redox status are a conspicuous feature of immune responses in a variety of eukaryotes, but the associated signalling mechanisms are not well understood. In plants, attempted microbial infection triggers the rapid synthesis of nitric oxide and a parallel accumulation of reactive oxygen intermediates, the latter generated by NADPH oxidases related to those responsible for the pathogen-activated respiratory burst in phagocytes. Both nitric oxide and reactive oxygen intermediates have been implicated in controlling the hypersensitive response, a programmed execution of plant cells at sites of attempted infection. However, the molecular mechanisms that underpin their function and coordinate their synthesis are unknown. Here we show genetic evidence that increases in cysteine thiols modified using nitric oxide, termed S-nitrosothiols, facilitate the hypersensitive response in the absence of the cell death agonist salicylic acid and the synthesis of reactive oxygen intermediates. Surprisingly, when concentrations of S-nitrosothiols were high, nitric oxide function also governed a negative feedback loop limiting the hypersensitive response, mediated by S-nitrosylation of the NADPH oxidase, AtRBOHD, at Cys 890, abolishing its ability to synthesize reactive oxygen intermediates. Accordingly, mutation of Cys 890 compromised S-nitrosothiol- mediated control of AtRBOHD activity, perturbing the magnitude of cell death development. This cysteine is evolutionarily conserved and specifically S-nitrosylated in both human and fly NADPH oxidase, suggesting that this mechanism may govern immune responses in both plants and animals.

Download File

PDF (Abstract)
S-nitrosylation of NADPH oxidase regulates cell death in plant immunity.pdf

Download (38kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1038/nature10427
Publisher: Macmillan Publishers
Keywords: Plant sciences; Molecular biology; Genetics; Genomics; Chemistry
Depositing User: Nabilah Mustapa
Date Deposited: 15 Sep 2015 00:56
Last Modified: 15 Sep 2015 00:56
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1038/nature10427
URI: http://psasir.upm.edu.my/id/eprint/40256
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item