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Development of a highly specific recombinant Toxocara canis second-stage larva excretory-secretory antigen for immunodiagnosis of human toxocariasis


Citation

Yamasaki, Hiroshi and Araki, Kunioki and Lim, Patricia Kim Chooi and Zasmy, Ngah and Mak, Joon Wah and Taib, Radzan and Aoki, Takashi (2000) Development of a highly specific recombinant Toxocara canis second-stage larva excretory-secretory antigen for immunodiagnosis of human toxocariasis. Journal of Clinical Microbiology, 38 (4). pp. 1409-1413. ISSN 0095-1137; ESSN: 1098-660X

Abstract

The specificity of the recombinant Toxocara canisantigen developed for the immunodiagnosis of human toxocariasis was compared with that of the excretory-secretory antigen from T. canis second-stage larvae (TES) by enzyme-linked immunosorbent assay. A total of 153 human serum samples from patients infected with 20 different helminths, including 11 cases of toxocariasis, were examined. No false-negative reactions were observed for the toxocariasis cases. When the TES was used at concentrations of 0.5 and 0.125 μg/ml, cross-reactions were observed in 79 (55.6%) and 61 (43.0%) of 142 cases, respectively. In contrast, when the recombinant antigen was tested at a concentration of 0.5 μg/ml, cross-reactions were observed in 19 (13.4%) of 142 cases. At a concentration of 0.125 μg/ml, however, the cross-reaction rate decreased sharply to only 2.1%, corresponding to 3 of 142 cases. The cross-reactions occurred with one case each of gnathostomiasis, paragonimiasis withParagonimus miyazakii, and spirometriasis, in which high antibody titers were detected. In addition, the recombinant antigen showed negative reactions with serum samples from patients infected with Ascaris and hookworms, which are the most common parasites in the world. These findings are also supported by experiments with animals infected with Ascaris and hookworm. From these results, the recombinant antigen is highly specific for toxocariasis and may provide more reliable diagnostic results than other methods.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Publisher: American Society for Microbiology
Keywords: Toxocara canis; Toxocariasis; Recombinant antigen
Depositing User: Nabilah Mustapa
Date Deposited: 27 Aug 2015 03:35
Last Modified: 27 Aug 2015 03:35
URI: http://psasir.upm.edu.my/id/eprint/40049
Statistic Details: View Download Statistic

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