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A simple technique for the study of fish meiotic chromosomes


Bongso, Tuan Ariffeen and Mohammad Mohsin, Abu Khair and Siraj, Siti Shapor (1982) A simple technique for the study of fish meiotic chromosomes. Malaysian Applied Biology, 11 (1). pp. 47-49. ISSN 0126-8643


A simple technique to study meiotic chromosomes from the testes of fish injected with colchicine is described. The minced testes was treated with a hypotonic solution of 0.075 M potassium chloride followed by fixation with Carnoy's solution. Air dried slides containing three to four drops of cell suspension were stained with 2 per cent aceto-orcein to display the various stages of meiosis. Clarity of chromosomes and adequate numbers of metaphase spreads per sample were obtained. Satisfactory results were also achieved for mitotic chromosomes when the same technique was used on gill preparations

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Additional Metadata

Item Type: Article
Divisions: Universiti Pertanian Malaysia
Faculty of Fisheries and Marine Science
Faculty of Veterinary Medicine
Publisher: Malaysian Society of Applied Biology
Keywords: Technique; Fish; Meiotic chromosomes
Depositing User: Azhar Abdul Rahman
Date Deposited: 11 Aug 2015 01:47
Last Modified: 11 Aug 2015 01:47
URI: http://psasir.upm.edu.my/id/eprint/39525
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