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Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor


Citation

Mel, Maizirwan and Abdul Rahman, Abdul Rafiz and Hasim, Yumi Hasyun and Ali, Abdul Manaf (2005) Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor. In: International Advanced Technology Congress: Conference on Computer Integrated Systems, 6 - 8 Dec. 2005, Putrajaya, Malaysia. .

Abstract / Synopsis

A study was done to determine the viability improvement of hybridoma cell producing monoclonal antibody. Using the optimized media, the RC1 hybridoma cells were cultured in T-Flask, MiniPerm bioreactor, and stirred tank bioreactor. The study show that the performance of hybridoma cells growth in the three different culture systems has revealed that in stirred tank bioreactor gave the highest cell percentage of viability (> 90% for five days) and the highest specific growth rate. The growth kinetic values for specific growth rate (μ) obtained from T-Flask, MiniPerm and stirred bioreactor were 0.0151, 0.0243 and 0.0289h-1, respectively. While, the values of doubling time (td) obtained from T-Flask, MiniPerm and stirred bioreactor were 46, 29, and 24h, respectively.


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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Keywords: Monoclonal antibody; Hybridoma cell; Bioreactor
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 16 Jun 2015 10:45
Last Modified: 16 Jun 2015 10:45
URI: http://psasir.upm.edu.my/id/eprint/38791
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