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Annexin II as a dengue virus serotype 2 interacting protein mediating virus interaction on Vero cells


Citation

Aliyu, Isah Abubakar and Ling, King Hwa and Md Hashim, Nur Fariesha and Lam, Jia Yong and Chee, Hui Yee (2019) Annexin II as a dengue virus serotype 2 interacting protein mediating virus interaction on Vero cells. Viruses, 11 (4). art. no. 335. pp. 1-12. ISSN 1999-4915

Abstract

Recent evidence has demonstrated that dengue virus requires active filopodia formation for a successful infection. However, the cellular factor involved in the interaction has not been fully elucidated. We used a combination of virus overlay protein binding assay and LC-MS/MS, and identified annexin II as a dengue virus serotype 2 (DENV2) interacting protein on Vero cells, upon filopodia induction. Flow cytometry analysis showed annexin II on the Vero cells surface increased when DENV2 was added. The amount of annexin II in the plasma membrane fraction was reduced as the infection progressed. Antibody-mediated inhibition of infection and siRNA-mediated knockdown of annexin II expression significantly reduced DENV2 infection and production levels. Collectively, we demonstrated that annexin II is one of the host factor involved in DENV2 binding on Vero cells.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.3390/v11040335
Publisher: MDPI
Keywords: Dengue; Annexin II; Vero; siRNA; Antibody-mediated inhibition
Depositing User: Nabilah Mustapa
Date Deposited: 04 May 2020 16:47
Last Modified: 04 May 2020 16:47
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/v11040335
URI: http://psasir.upm.edu.my/id/eprint/38409
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