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Multiple antibiotic resistance (MAR), plasmid profiles, and DNA polymorphisms among Vibrio vulnificus isolates


Al-Dulaimi, Mohammed M. Kurdi and Abd Mutalib, Sahilah and Abd Ghani, Maa'ruf and Mohd Zaini, Nurul Aqilah and Ariffin, Ahmad Azuhairi (2019) Multiple antibiotic resistance (MAR), plasmid profiles, and DNA polymorphisms among Vibrio vulnificus isolates. Antibiotics, 8 (2). art. no. 68. pp. 1-13. ISSN 2079-6382


Sixty strains (n = 60) of Vibrio vulnificus were examined for their multiple antibiotic resistance (MAR) index, plasmid profiles, and DNA polymorphisms. Thirty-seven strains (n = 37) were isolated from cockles (Anadara granosa) in Malaysia, while 23 (n = 23) isolates were isolated from clams (Mercenaria mercenaria) in Qatar. All isolates were resistant to two or more of the antibiotics tested, with the most common resistances were demonstrated towards penicillin (93%), ampicillin (70%), cephalothin (65%), clindamycin (66%), vancomycin (64%), and erythromycin (51%). The antibiotic that experienced the least resistance was kanamycin (6%), and all isolates were susceptible to cefoperazone, streptomycin, and tetracycline. The MAR index for the V. vulnificus isolated from Malaysia and Qatar, possessed similar values which ranged from 0.2 to 0.7, respectively. Plasmid analysis demonstrated that 65% of V. vulnificus strains harbored plasmids, while 35% were not. Nineteen (P1–P19) plasmids profiles were observed. No specific cluster or group was observed although they were isolated from different sample sources and locations by phylogenetic analysis using GelCompar II software at an 80% similarity level. Results demonstrated the high MAR index and genomic heterogeneity of V. vulnificus, which are of great concern to the human health of those who have consumed cockles and clams from the study area.

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Official URL or Download Paper: https://www.mdpi.com/2079-6382/8/2/68

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.3390/antibiotics8020068
Publisher: MDPI
Keywords: Multiple antibiotic resistance (MAR) index; Plasmid profiles; DNA polymorphisms; Vibrio vulnificus; Genomic heterogeneity
Depositing User: Nabilah Mustapa
Date Deposited: 04 May 2020 16:23
Last Modified: 04 May 2020 16:23
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/antibiotics8020068
URI: http://psasir.upm.edu.my/id/eprint/38361
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