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An influenza A vaccine based on the extracellular domain of matrix 2 protein protects BALB/C mice against H1N1 and H3N2


Citation

Ong, Hui Kian and Yong, Chean Yeah and Tan, Wen Siang and Yeap, Swee Keong and Omar, Abdul Rahman and Abdul Razak, Mariatulqabtiah and Ho, Kok Lian (2019) An influenza A vaccine based on the extracellular domain of matrix 2 protein protects BALB/C mice against H1N1 and H3N2. Vaccines, 7 (3). art. no. 91. pp. 1-15. ISSN 2076-393X

Abstract

Current seasonal influenza A virus (IAV) vaccines are strain-specific and require annual reconstitution to accommodate the viral mutations. Mismatches between the vaccines and circulating strains often lead to high morbidity. Hence, development of a universal influenza A vaccine targeting all IAV strains is urgently needed. In the present study, the protective efficacy and immune responses induced by the extracellular domain of Matrix 2 protein (M2e) displayed on the virus-like particles of Macrobrachium rosenbergii nodavirus (NvC-M2ex3) were investigated in BALB/c mice. NvC-M2ex3 was demonstrated to be highly immunogenic even in the absence of adjuvants. Higher anti-M2e antibody titers corresponded well with increased survival, reduced immunopathology, and morbidity of the infected BALB/c mice. The mice immunized with NvC-M2ex3 exhibited lower H1N1 and H3N2 virus replication in the respiratory tract and the vaccine activated the production of different antiviral cytokines when they were challenged with H1N1 and H3N2. Collectively, these results suggest that NvC-M2ex3 could be a potential universal influenza A vaccine.


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Official URL or Download Paper: https://www.mdpi.com/2076-393X/7/3/91

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Medicine and Health Science
Faculty of Veterinary Medicine
Institute of Bioscience
DOI Number: https://doi.org/10.3390/vaccines7030091
Publisher: MDPI
Keywords: Universal influenza A vaccine; Macrobrachium rosenbergii nodavirus; Extracellular domain of matrix 2 protein; Virus-like particle; H1N1; H3N2
Depositing User: Nabilah Mustapa
Date Deposited: 04 May 2020 16:12
Last Modified: 04 May 2020 16:12
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/vaccines7030091
URI: http://psasir.upm.edu.my/id/eprint/38287
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