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Aqueous partition of methanolic extract of Dicranopteris linearis leaves protects against liver damage induced by paracetamol


Zakaria, Zainul Amiruddin and Kamisan, Farah Hidayah and Mohd. Nasir, Nurliana and Teh, Lay Kek and Salleh, Mohd Zaki (2019) Aqueous partition of methanolic extract of Dicranopteris linearis leaves protects against liver damage induced by paracetamol. Nutrients, 11 (12). art. no. 2945. pp. 1-19. ISSN 2072-6643


This study aimed to determine the antioxidant and hepatoprotective activities of semi-purified aqueous partition obtained from the methanol extract of Dicranopteris linearis (AQDL) leaves against paracetamol (PCM)-induced liver intoxication in rats. The test solutions, AQDL (50, 250, and 500 mg/kg), were administered orally to rats (n = 6) once daily for seven consecutive days followed by the hepatotoxicity induction using 3 g/kg PCM (p.o.). Blood was collected for serum biochemical parameters analysis while the liver was collected for histopathological examination and endogenous antioxidant enzymes analysis. AQDL was also subjected to antioxidant determination and phytochemical analysis. Results obtained show that AQDL possessed high total phenolic content (TPC) value and remarkable radical scavenging activities. AQDL also significantly (p < 0.05) reduced the liver weight/body weight (LW/BW) ratio or serum level of ALT, AST, and total bilirubin while significantly (p < 0.05) increase the level of superoxide dismutase (SOD) and catalase (CAT) without affecting the malondialdehyde (MDA) in the liver indicating its hepatoprotective effect. Phytoconstituents analyses showed only the presence of saponins and triterpenes, but lack of flavonoids. In conclusion, AQDL exerts hepatoprotective activity via its high antioxidant potential and ability to modulate the endogenous enzymatic antioxidant defense system possibly via the synergistic action of saponins and triterpenes.

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Official URL or Download Paper: https://www.mdpi.com/2072-6643/11/12/2945

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.3390/nu11122945
Publisher: MDPI
Keywords: Dicranopteris linearis; Hepatoprotective; Free radical scavenging activity; Endogenous antioxidant enzymes; Saponins; Triterpenes; Phenolic derivatives
Depositing User: Nabilah Mustapa
Date Deposited: 04 May 2020 15:58
Last Modified: 04 May 2020 15:58
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/nu11122945
URI: http://psasir.upm.edu.my/id/eprint/38213
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