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Probiotic properties of Bacillus strains isolated from stingless bee (Heterotrigona itama) honey collected across Malaysia


Citation

Zulkhairi Amin, Fatin Aina and Sabri, Suriana and Ismail, Maznah and Chan, Kim Wei and Ismail, Norsharina and Mohd Esa, Norhaizan and Mohd Lila, Mohd Azmi and Zawawi, Norhasnida (2020) Probiotic properties of Bacillus strains isolated from stingless bee (Heterotrigona itama) honey collected across Malaysia. International Journal of Environmental Research and Public Health, 17 (1). art. no. 278. pp. 1-15. ISSN 1661-7827; ESSN: 1660-4601

Abstract

This study aimed to isolate, identify, and evaluate the probiotic properties of Bacillus species from honey of the stingless bee Heterotrigona itama. Bacillus spp. were isolated from five different H. itama meliponicultures, and the isolates were characterized through Gram-staining and a catalase test. Tolerance to acidic conditions and bile salt (0.3%), hydrophobicity, and autoaggregation tests were performed to assess the probiotic properties of the selected isolates, B. amyloliquefaciens HTI-19 and B. subtilis HTI-23. Both Bacillus isolates exhibited excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria and possessed significantly high survival rates in 0.3% bile solution for 3 h. Their survival rates in acidic conditions were also comparable to a commercial probiotic strain, Lactobacillus rhamnosus GG. Interestingly, the hydrophobicity and autoaggregation percentage showed no significant difference from L. rhamnosus GG, a commercial probiotic strain. The results from this study suggest that B. amyloliquefaciens HTI-19 and B. subtilis HTI-23 isolated from stingless bee honey have considerably good probiotic properties. Therefore, more studies should be done to investigate the effects of these bacteria cultures on gastrointestinal health.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Food Science and Technology
Faculty of Medicine and Health Science
Faculty of Veterinary Medicine
Institute of Bioscience
DOI Number: https://doi.org/10.3390/ijerph17010278
Publisher: MDPI
Keywords: Stingless bee honey; Probiotic Bacillus strains; Molecular identification; Antimicrobial activity; Pathogenic bacteria
Depositing User: Nabilah Mustapa
Date Deposited: 14 Apr 2020 13:58
Last Modified: 14 Apr 2020 13:58
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/ijerph17010278
URI: http://psasir.upm.edu.my/id/eprint/38104
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