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In vitro antioxidant and in vivo hepatoprotective effect on ethanol-mediated liver damage of spray dried Vernonia amygdalina water extract


Citation

Ho, Wan Yong and Yeap, Swee Keong and Liang, Woon San and Beh, Boon Kee and Mohamad, Nurul Elyani and Mohamed Alitheen, Noorjahan Banu (2015) In vitro antioxidant and in vivo hepatoprotective effect on ethanol-mediated liver damage of spray dried Vernonia amygdalina water extract. Pakistan Journal of Pharmaceutical Sciences, 28 (1). pp. 15-22. ISSN 1011-601X

Abstract

Vernonia amygdalina is a strong natural antioxidant that possessed various medicinal properties. In this study, the spray-dried water extract of V. amygdalina was evaluated for its in vitro antioxidant capacity and in vivo hepatoprotective effect against alcoholic-mediated liver damage. Total phenolic and flavonoid content of spray-dried V. amygdalina water extract were determined. Liver enzyme profiles, liver antioxidant level and nitric oxide level were evaluated in alcohol-induced liver injured mice or co-supplement with spray-dried V. amydalina. Water extract of spraydried V. amygalina that contained phenolic content of 24.8±1.5 mg/g gallic acid equivalent and total flavonoid content of 25.7±1.3 mg/g catechin equivalent was able to inhibit 50% of xanthine and tyrosinase oxidation at 170 µg/mL and 2 mg/mL, respectively. On the other hand, extracts at both 10 and 50 mg/kg body weight were able to reduce the levels of Alanine transaminase (ALT), Alkaline phosphatase (ALP), Aspartate transaminase (AST), triglyceride and total bilirubin content inthe alcohol-mediated liver injury in mice. Furthermore, it also helped to increase levels of Superoxide dismutase (SOD), Ferric reducing ability of plasma (FRAP) and reduce the levels of Nitric oxide (NO) and Malondialdehyde (MDA) in the liver of the treated mice. These resultssuggestedthat water extract of spray-dried V. amygdalina exhibited liver protective effect, which could be contributed by its antioxidant properties.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
Publisher: Faculty of Pharmacy, University of Karachi
Keywords: Spray-dried water extract; Phenolic; flavonoid; Alcohol; Liver damage
Depositing User: Nabilah Mustapa
Date Deposited: 15 Dec 2015 03:41
Last Modified: 15 Dec 2015 03:41
URI: http://psasir.upm.edu.my/id/eprint/38003
Statistic Details: View Download Statistic

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