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In vitro antioxidant and antiproliferative activities of methanolic plant part extracts of Theobroma cacao


Baharum, Zainal and Md. Akim, Abdah and Yap, Taufiq Yun Hin and Abd Hamid @ Abdul Razak, Roslida and Kasran, Rosmin (2014) In vitro antioxidant and antiproliferative activities of methanolic plant part extracts of Theobroma cacao. Molecules, 19 (11). pp. 18317-18331. ISSN 1420-3049


The aims of this study were to determine the antioxidant and antiproliferative activity of the following Theobroma cacao plant part methanolic extracts: leaf, bark, husk, fermented and unfermented shell, pith, root, and cherelle. Antioxidant activity was determined using 2,2-diphenyl-2-picrylhydrazyl (DPPH), thiobarbituric acid-reactive substances (TBARS), and Folin-Ciocalteu assays; the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) assay was used to determine antiproliferative activity. The root extract had the highest antioxidant activity; its median effective dose (EC50) was 358.3 ± 7.0 µg/mL and total phenolic content was 22.0 ± 1.1 g GAE/100 g extract as compared to the other methanolic plant part extracts. Only the cherelle extract demonstrated 10.4% ± 1.1% inhibition activity in the lipid peroxidation assay. The MTT assay revealed that the leaf extract had the highest antiproliferative activity against MCF-7 cells [median inhibitory concentration (IC50) = 41.4 ± 3.3 µg/mL]. Given the overall high IC50 for the normal liver cell line WRL-68, this study indicates that T. cacao methanolic extracts have a cytotoxic effect in cancer cells, but not in normal cells. Planned future investigations will involve the purification, identification, determination of the mechanisms of action, and molecular assay of T. cacao plant extracts.

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Official URL or Download Paper: http://www.mdpi.com/1420-3049/19/11/18317

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Faculty of Science
DOI Number: https://doi.org/10.3390/molecules191118317
Publisher: MDPI
Keywords: Antioxidant; Antiproliferative; Natural product; Phenolics; Plant; Theobroma cacao
Depositing User: Nabilah Mustapa
Date Deposited: 18 Dec 2015 01:54
Last Modified: 31 Oct 2016 02:02
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules191118317
URI: http://psasir.upm.edu.my/id/eprint/38002
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