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Immunomodulatory effects of Newcastle disease virus AF2240 strain on human peripheral blood mononuclear cells


Citation

Lam, Han Yuen and Yusoff, Khatijah and Yeap, Swee Keong and Subramani, Tamilselvan and Abd. Aziz, Suraini and Omar, Abdul Rahman and Mohamed Alitheen, Noorjahan Banu (2014) Immunomodulatory effects of Newcastle disease virus AF2240 strain on human peripheral blood mononuclear cells. International Journal of Medical Sciences, 11 (12). pp. 1240-1247. ISSN 1449-1907

Abstract

Immunotherapy has raised the attention of many scientists because it hold promise to be an attractive therapeutic strategy to treat a number of disorders. In this study, the immunomodulatory effects of low titers of Newcastle disease virus (NDV) AF2240 on human peripheral blood mononuclear cells (PBMC) were analyzed. We evaluated cytokine secretion and PBMC activation by cell proliferation assay, immunophenotyping and enzyme linked immunosorbent assay. The proliferation of the human PBMC was measured to be 28.5% and 36.5% upon treatment with 8 hemaglutinin unit (HAU) and 2 HAU of NDV respectively. Interestingly, the percentage of cells with activating markers CD16 and CD56 were increased significantly. Furthermore, the intracellular perforin and granzyme levels were also increased upon virus infection. Human PBMC treated with NDV titer 8 HAU was found to stimulate the highest level of cytokine production including interferon-γ, interleukin-2 and interleukin-12. The release of these proteins contributes to the antitumor effect of PBMC against MCF-7 breast cancer cells. Based on the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay, activated human PBMC showed high cytolytic efficiency towards human breast tumor cells. In summary, NDV was able to stimulate PBMC proliferation, cytokine secretion and cytolytic activity.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
DOI Number: https://doi.org/10.7150/ijms.8170
Publisher: Ivyspring International Publisher
Keywords: AF2240; Breast cancer; Immunomodulation; Immunophenotyping; Newcastle disease virus; PBMC
Depositing User: Nabilah Mustapa
Date Deposited: 29 Dec 2015 12:25
Last Modified: 29 Dec 2015 12:25
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.7150/ijms.8170
URI: http://psasir.upm.edu.my/id/eprint/37936
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