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Genotypic characterization of Streptococcus pneumoniae serotype 19F in Malaysia


Jeevajothi Nathan, Jayakayatri and Mohd Desa, Mohd Nasir and Thong, Kwai Lin and Clarke, Stuart C. and Masri, Siti Norbaya and Md Yasin, Rohani and Mohd Taib, Niazlin (2014) Genotypic characterization of Streptococcus pneumoniae serotype 19F in Malaysia. Infection, Genetics and Evolution, 21. pp. 391-394. ISSN 1567-1348; ESSN: 1567-7257


Streptococcus pneumoniae is an epidemiologically important bacterial pathogen. Recently, we reported the antibiotic susceptibility patterns of a limited collection of pneumococcal isolates in Malaysia with a high prevalence of erythromycin resistant strains. In the present study, 55 of the pneumococcal isolates of serotype 19F were further analysed by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The generated genotypic patterns were then correlated with the antibiograms previously reported. Forty-seven different PFGE profiles (PTs) were obtained, showing that the isolates were genetically diverse. MLST identified 16 sequence types (STs) with ST-236 being predominant (58.2%), followed by ST-81 (10.3%). Among the ST-236 isolates, 22 were erythromycin resistant S. pneumoniae (ERSP) and 15 were trimethoprim/sulfamethoxazole (TMP/SMX) resistant, while among ST-81, four isolates were ERSP and two were TMP/SMX resistant. The high prevalence of erythromycin resistant serotype 19F isolates of ST-236 in this study has also been reported in other North and South East Asian countries.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1016/j.meegid.2013.11.026
Publisher: Elsevier
Keywords: Streptococcus pneumoniae; Serotype 19F; Antibiogram; Multilocus sequence type; Pulsed field gel electrophoresis
Depositing User: Nabilah Mustapa
Date Deposited: 22 Apr 2016 02:36
Last Modified: 22 Apr 2016 02:36
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.meegid.2013.11.026
URI: http://psasir.upm.edu.my/id/eprint/37767
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