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Evaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice


Citation

Goh, Jun Zheng and Tang, Sook Nai and Chiong, Hoe Siong and Yong, Yoke Keong and Ahmad, Zuraini and Abdullah, Muhammad Nazrul Hakim (2015) Evaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice. International Journal of Nanomedicine, 10. pp. 297-303. ISSN 1178-2013

Abstract / Synopsis

Diclofenac is a nonsteroidal anti-inflammatory drug (NSAID) that exhibits anti-inflammatory, antinociceptive, and antipyretic activities. Liposomes have been shown to improve the therapeutic efficacy of encapsulated drugs. The present study was conducted to compare the antinociceptive properties between liposome-encapsulated and free-form diclofenac in vivo via different nociceptive assay models. Liposome-encapsulated diclofenac was prepared using the commercialized proliposome method. Antinociceptive effects of liposome-encapsulated and free-form diclofenac were evaluated using formalin test, acetic acid-induced abdominal writhing test, Randall–Selitto paw pressure test, and plantar test. The results of the writhing test showed a significant reduction of abdominal constriction in all treatment groups in a dose-dependent manner. The 20 mg/kg liposome-encapsulated diclofenac demonstrated the highest antinociceptive effect at 78.97% compared with 55.89% in the free-form group at equivalent dosage. Both liposome-encapsulated and free-form diclofenac produced significant results in the late phase of formalin assay at a dose of 20 mg/kg, with antinociception percentages of 78.84% and 60.71%, respectively. Significant results of antinociception were also observed in both hyperalgesia assays. For Randall–Sellito assay, the highest antinociception effect of 71.38% was achieved with 20 mg/kg liposome-encapsulated diclofenac, while the lowest antinociceptive effect of 17.32% was recorded with 0 mg/kg liposome formulation, whereas in the plantar test, the highest antinociceptive effect was achieved at 56.7% with 20 mg/kg liposome-encapsulated diclofenac, and the lowest effect was shown with 0 mg/kg liposome formulation of 8.89%. The present study suggests that liposome-encapsulated diclofenac exhibits higher antinociceptive efficacy in a dose-dependent manner in comparison with free-form diclofenac.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Halal Products Research Institute
DOI Number: https://doi.org/10.2147/IJN.S75545
Publisher: Dove Medical Press
Keywords: Antinociceptive; Diclofenac; Efficacy; Liposome; Nanoencapsulation
Depositing User: Nabilah Mustapa
Date Deposited: 18 Dec 2015 10:50
Last Modified: 18 Dec 2015 10:50
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.2147/IJN.S75545
URI: http://psasir.upm.edu.my/id/eprint/37519
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