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Determination of diagnostic value of Toxoplasma gondii recombinant surface antigen (SAG1, P30) in mouse experimental model


Citation

Abdullah, Wan Omar and Unyah, Ngah Zasmy and Awang Hamat, Rukman and Ithoi, Init and Basir, Rusliza and Abdul Ghani, Mohamed Kamel (2014) Determination of diagnostic value of Toxoplasma gondii recombinant surface antigen (SAG1, P30) in mouse experimental model. Malaysian Journal of Veterinary Research, 5 (1). pp. 83-88. ISSN 2180-3897

Abstract / Synopsis

The aim of this study was to test the potential diagnostic usefulness of recombinant Toxoplasma gondii SAG1 antigen and excretory-secretory antigen (ESA), with respect to toxoplasmosis detection and infection phase distinction in laboratory mouse by determining specifi c serum IgM and IgG antibodies with the use of indirect ELISA technique. The highest titre at the beginning of infection was demonstrated by immunoglobulin M while the highest titre at the end of the infection was displayed by immunoglobulin G. In contrast, sera of chronically infected mice, positive IgG titre was detected on day 14 p.i. for ESA or day seven p.i. with rSAG1 and increased thereafter until day 70 p.i. after which the titre stabilized. IgA antibody also showed similar kinetics as IgG. Potentially rSAG1 may be a suitable diagnostic antigen than ESA in the diagnosis of acute and chronic toxoplasmosis.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Publisher: Department of Veterinary Services Malaysia
Notes: Short communication
Keywords: Toxoplasmosis; Diagnosis; Recombinant; Surface antigen
Depositing User: Nabilah Mustapa
Date Deposited: 18 Aug 2015 13:13
Last Modified: 24 Aug 2015 08:10
URI: http://psasir.upm.edu.my/id/eprint/37024
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