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Cellular metabolic profiling of CrFK cells infected with feline infectious peritonitis virus using phenotype microarrays


Ng, Shing Wei and Selvarajah, Gayathri Thevi and Cheah, Yoke Kqueen and Mustaffa Kamal, Farina and Omar, Abdul Rahman (2020) Cellular metabolic profiling of CrFK cells infected with feline infectious peritonitis virus using phenotype microarrays. Pathogens, 9 (5). art. no. 412. pp. 1-12. ISSN 2076-0817


Feline infectious peritonitis (FIP) is a fatal feline immune-mediated disease caused by feline infectious peritonitis virus (FIPV). Little is known about the biological pathways associated in FIP pathogenesis. This is the first study aiming to determine the phenotypic characteristics on the cellular level in relation to specific metabolic pathways of importance to FIP pathogenesis. Methods: The internalization of type II FIPV WSU 79-1146 in Crandell-Rees Feline Kidney (CrFK) cells was visualized using a fluorescence microscope, and optimization prior to phenotype microarray (PM) study was performed. Then, four types of Biolog Phenotype MicroArray™ plates (PM-M1 to PM-M4) precoated with different carbon and nitrogen sources were used to determine the metabolic profiles in FIPV-infected cells. Results: The utilization of palatinose was significantly low in FIPV-infected cells; however, there were significant increases in utilizing melibionic acid, L-glutamine, L-glutamic acid and alanyl-glutamine (Ala-Gln) compared to non-infected cells. Conclusion: This study has provided the first insights into the metabolic profiling of a feline coronavirus infection in vitro using PMs and deduced that glutamine metabolism is one of the essential metabolic pathways for FIPV infection and replication. Further studies are necessary to develop strategies to target the glutamine metabolic pathway in FIPV infection.

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Official URL or Download Paper: https://www.mdpi.com/2076-0817/9/5/412

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Faculty of Veterinary Medicine
Institute of Bioscience
DOI Number: https://doi.org/10.3390/pathogens9050412
Publisher: MDPI
Keywords: Feline infectious peritonitis virus; Cellular metabolism; Phenotype microarray; Metabolic profiling; Glutamine; CrFK cells
Depositing User: Nabilah Mustapa
Date Deposited: 06 Jul 2020 03:32
Last Modified: 06 Jul 2020 03:32
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/pathogens9050412
URI: http://psasir.upm.edu.my/id/eprint/36790
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