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Characterisation of L. monocytogenes strains isolated from salad vegetables


Ponniah, Jeyaletchumi and Robin, Tunung and Radu, Son and Cheah, Yoke Kqueen and Mohamad Ghazali, Farinazleen and Nishibuchi, Mitsuaki and Nakaguchi, Yoshitsugu and Malakar, Pradeep (2015) Characterisation of L. monocytogenes strains isolated from salad vegetables. International Journal of Food Safety, Nutrition and Public Health, 5 (2). pp. 137-150. ISSN 1479-3911; ESSN:1479-392X


Seventy-one strains of L. monocytogenes isolated from raw salad vegetables consumed in the minimally processed state in Malaysia were characterised by serotyping, random amplified polymorphic DNA-polymerase chain reaction analysis (RAPD-PCR) and antimicrobial susceptibility testing. Based on serotyping, only one strain belonged to serotype 4 while the rest were from serotype 1. RAPD-PCR typing was carried out using two primers, i.e., OPAR-8 and GEN 1-50-09. Based on RAPD-PCR fingerprinting, the strains could be grouped into six composite profile groups and a solitary isolate. The isolates were also evaluated against 16 antibiotics. Excluding nalidixic acid and oxacillin for which L. monocytogenes has natural resistance, the highest prevalence of resistance was against cephalexin. None of the isolates were resistant against imipenem and kanamycin. The multiple antibiotic resistance index (MARI) ranged from 0.06 to 0.63. It was found that most (82%) isolates were susceptible to antibiotics commonly used in veterinary and human listeriosis treatment.

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Additional Metadata

Item Type: Article
Divisions: Centre of Excellence in Food Safety
Faculty of Food Science and Technology
Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1504/IJFSNPH.2015.067562
Publisher: Inderscience Publishers
Keywords: L. monocytogenes; Raw vegetables; Salad vegetables; Serotyping; RAPD; Random amplified polymorphic DNA; Polymerase chain reaction; PCR; Antimicrobial Susceptibility; Malaysia; Antibiotics; Antibiotic resistance
Depositing User: Nabilah Mustapa
Date Deposited: 05 Aug 2015 03:25
Last Modified: 28 Oct 2015 02:38
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1504/IJFSNPH.2015.067562
URI: http://psasir.upm.edu.my/id/eprint/36758
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