UPM Institutional Repository

Cartilage regeneration by chondrogenic induced adult stem cells in osteoarthritic sheep model


Ude, Chinedu C. and Sulaiman, Shamsul and Ng, Min Hwei and Chen, Hui Cheng and Ahmad, Johan and Mohamad Yahaya, Nor Hamdan and Saim, Aminuddin and Idrus, Ruszymah (2014) Cartilage regeneration by chondrogenic induced adult stem cells in osteoarthritic sheep model. PLOS ONE, 9 (6). art. no. e98770. pp. 1-13. ISSN 1932-6203


Objectives: In this study, Adipose stem cells (ADSC) and bone marrow stem cells (BMSC), multipotent adult cells with the potentials for cartilage regenerations were induced to chondrogenic lineage and used for cartilage regenerations in surgically induced osteoarthritis in sheep model. Methods: Osteoarthritis was induced at the right knee of sheep by complete resection of the anterior cruciate ligament and medial meniscus following a 3-weeks exercise regimen. Stem cells from experimental sheep were culture expanded and induced to chondrogenic lineage. Test sheep received a single dose of 2×107 autologous PKH26-labelled, chondrogenically induced ADSCs or BMSCs as 5 mls injection, while controls received 5 mls culture medium. Results: The proliferation rate of ADSCs 34.4±1.6 hr was significantly higher than that of the BMSCs 48.8±5.3 hr (P = 0.008). Chondrogenic induced BMSCs had significantly higher expressions of chondrogenic specific genes (Collagen II, SOX9 and Aggrecan) compared to chondrogenic ADSCs (P = 0.031, 0.010 and 0.013). Grossly, the treated knee joints showed regenerated de novo cartilages within 6 weeks post-treatment. On the International Cartilage Repair Society grade scores, chondrogenically induced ADSCs and BMSCs groups had significantly lower scores than controls (P = 0.0001 and 0.0001). Fluorescence of the tracking dye (PKH26) in the injected cells showed that they had populated the damaged area of cartilage. Histological staining revealed loosely packed matrixes of de novo cartilages and immunostaining demonstrated the presence of cartilage specific proteins, Collagen II and SOX9. Conclusion: Autologous chondrogenically induced ADSCs and BMSCs could be promising cell sources for cartilage regeneration in osteoarthritis.

Download File

PDF (Abstract)
Cartilage regeneration by chondrogenic induced adult stem cells in osteoarthritic sheep model.pdf

Download (85kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.1371/journal.pone.0098770
Publisher: Public Library of Science
Keywords: Cartilage regenerations; Chondrogenically; Adipose stem cells (ADSC); Bone marrow stem cells (BMSC); Osteoarthritis
Depositing User: Nabilah Mustapa
Date Deposited: 25 Jul 2015 23:57
Last Modified: 06 Oct 2015 00:47
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1371/journal.pone.0098770
URI: http://psasir.upm.edu.my/id/eprint/36710
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item