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Biochemical and molecular identification of Enterococcus spp. from red pitaya


Ong, Yien Yien and Tan, Wen Siang and Mohamad, Rosfarizan and Sieo, Chin Chin and Tey, Beng Ti (2014) Biochemical and molecular identification of Enterococcus spp. from red pitaya. Process Biochemistry, 49 (4). pp. 563-568. ISSN 1359-5113; ESSN: 1873-3298


Red pitaya is a popular fruit worldwide due to its unique appearance and high nutrient contents. Many studies about the nutritional content and the physico-chemical characteristics of red pitaya have been performed but microorganisms that occurred naturally in the fruit remained unknown. Therefore, this study was conducted to characterize the enterococci present in red pitaya. Enterococci were isolated from red pitaya fruit and characterized with biochemical tests, randomly amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) of the 16S rDNA region. A total of 30 bacteria were isolated from red pitaya, of which 20 isolates were confirmed as enterococci, genus Enterococcus. 13 isolates were further confirmed as Enterococcus casseliflavus and 7 were identified as Enterococcus gallinarum.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
DOI Number: https://doi.org/10.1016/j.procbio.2014.01.019
Publisher: Elsevier
Keywords: Red pitaya; Randomly amplified polymorphic DNA; Restriction fragment length polymorphism; Enterococcus
Depositing User: Nabilah Mustapa
Date Deposited: 04 Sep 2015 12:35
Last Modified: 04 Sep 2015 12:35
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.procbio.2014.01.019
URI: http://psasir.upm.edu.my/id/eprint/36644
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