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Antioxidant activity of phenolics-saponins rich fraction prepared from defatted kenaf seed meal


Chan, Kim Wei and Iqbal, Shahid and Khong, Nicholas Mun Hoe and Ooi, Der Jiun and Ismail, Maznah (2014) Antioxidant activity of phenolics-saponins rich fraction prepared from defatted kenaf seed meal. LWT - Food Science and Technology, 56 (1). pp. 181-186. ISSN 0023-6438


The current study is aimed to determine the antioxidant properties of crude ethanolic extract (CEE) of defatted kenaf seed meal (DKSM) and its derived n-butanol (BF) and aqueous (AqF) fractions. Spectrophotometric assays showed that BF contained the highest amount of phenolic compounds and saponins, followed by CEE and AqF (p < 0.05). Similarly, HPLC-DAD analysis revealed that level of all the detected predominant phenolic compounds was significantly higher in BF (p < 0.05). Through multiple antioxidant assays, BF exhibited higher antioxidant activity than CEE and AqF, except for iron chelating activity (p < 0.05). Antioxidant activity of CEE and fractions were strongly correlated to their phenolic and saponin contents. This study showed that phenolic compounds and saponins could be extracted and partially purified simultaneously from DKSM by employing a simple alcoholic extraction–fractionation procedure. High antioxidative phenolics–saponins rich fraction from DKSM is a potential active ingredient that could be applied in nutraceuticals, functional foods as well as natural food preservatives.

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Additional Metadata

Item Type: Article
Divisions: Institute of Bioscience
DOI Number: https://doi.org/10.1016/j.lwt.2013.10.028
Publisher: Elsevier
Keywords: Defatted kenaf seed meal; Hibiscus cannabinus L.; Phenolic compounds; Saponins; Antioxidant activity
Depositing User: Nabilah Mustapa
Date Deposited: 31 May 2015 02:05
Last Modified: 07 Sep 2015 06:43
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.lwt.2013.10.028
URI: http://psasir.upm.edu.my/id/eprint/36488
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