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Anticoagulant activity of polyphenolic-polysaccharides isolated from Melastoma malabathricum L.


Khoo, Li Teng and Abas, Faridah and Abdullah, Janna Ong and Mohd Tohit, Eusni Rahayu and Hamid, Muhajir (2014) Anticoagulant activity of polyphenolic-polysaccharides isolated from Melastoma malabathricum L. Evidence-Based Complementary and Alternative Medicine, 2014. art. no. 614273. pp. 1-8. ISSN 1741-427X; ESSN: 1741-4288


Melastoma malabathricum Linn. is a perennial traditional medicine plants that grows abundantly throughout Asian countries. In this study, M. malabathricum Linn. leaf hot water crude extract with anticoagulant activity was purified through solid phase extraction cartridge and examined for the bioactive chemical constituents on blood coagulation reaction. The SPE purified fractions were, respectively, designated as F1, F2, F3, and F4, and each was subjected to the activated partial thromboplastin time (APTT) anticoagulant assay. Active anticoagulant fractions (F1, F2, and F3) were subjected to chemical characterisation evaluation. Besides, neutral sugar for carbohydrate part was also examined. F1, F2, and F3 were found to significantly prolong the anticoagulant activities in the following order, F1 > F2 > F3, in a dose dependent manner. In addition, carbohydrate, hexuronic acid, and polyphenolic moiety were measured for the active anticoagulant fractions (F1, F2, and F3). The characterisation of chemical constituents revealed that all these three fractions contained acidic polysaccharides (rhamnogalacturonan, homogalacturonan, and rhamnose hexose-pectic type polysaccharide) and polyphenolics. Hence, it was concluded that the presence of high hexuronic acids and polysaccharides, as well as polyphenolics in traditional medicinal plant, M. malabathricum, played a role in prolonging blood clotting in the intrinsic pathway.

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Official URL or Download Paper: http://www.hindawi.com/journals/ecam/2014/614273/

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Food Science and Technology
Faculty of Medicine and Health Science
Institute of Bioscience
DOI Number: https://doi.org/10.1155/2014/614273
Publisher: Hindawi Publishing Corporation
Keywords: Melastoma malabathricum Linn.; Blood coagulation; Polyphenolic-polysaccharides; Anticoagulant
Depositing User: Nabilah Mustapa
Date Deposited: 14 Aug 2015 10:30
Last Modified: 17 Sep 2015 07:40
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2014/614273
URI: http://psasir.upm.edu.my/id/eprint/36471
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