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Analysis of human bradykinin receptor gene and endothelial nitric oxide synthase gene polymorphisms in end-stage renal disease among Malaysians


Citation

Vasudevan, Ramachandran and Ismail, Patimah and Jaafar, N. I. and Mohamad, N. A. and Etemad, E. and Wan Sulaiman, Wan Aliaa and Eshkor, S. (2014) Analysis of human bradykinin receptor gene and endothelial nitric oxide synthase gene polymorphisms in end-stage renal disease among Malaysians. Balkan Journal of Medical Genetics, 17 (1). pp. 37-40. ISSN 1311-0160; ESSN: 2199-5761

Abstract / Synopsis

The aim of this study was to determine the association of the c.894G>T; p.Glu298Asp polymorphism and the variable number tandem repeat (VNTR) polymorphism of the endothelial nitric oxide synthase (eNOS) gene and c.181C>T polymorphism of the bradykinin type 2 receptor gene (B2R) in Malaysian end-stage renal disease (ESRD) subjects. A total of 150 ESRD patients were recruited from the National Kidney Foundation’s (NKF)dialysis centers in Malaysia and compared with 150 normal healthy individuals. Genomic DNA was extracted from buccal cells of all the subjects. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was carried out to amplify the products and the restricted fragments were separated by agarose gel electrophoresis. Statistical analyses were carried out using software where a level of p <0.05 was considered to be statistically significant. The genotypic and allelic frequencies of the B2R gene (c.181C>T, 4b/a) and eNOS gene (c.894G>T) polymorphisms were not statistically significant (p >0.05) when compared to the control subjects. The B2R and eNOS gene polymorphisms may not be considered as genetic susceptibility markers for Malaysian ESRD subjects.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Institute of Gerontology
DOI Number: https://doi.org/10.2478/bjmg-2014-0023
Publisher: Walter de Gruyter GmbH
Keywords: Bradykinin type 2 receptor (B2R) gene; Endothelial nitric oxide synthase (eNOS) gene; End-stage renal disease (ESRD); Polymorphism
Depositing User: Nabilah Mustapa
Date Deposited: 13 Jun 2015 11:57
Last Modified: 07 Oct 2015 11:30
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.2478/bjmg-2014-0023
URI: http://psasir.upm.edu.my/id/eprint/36450
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