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Alpha-linolenic acid supplementation in Tris extender can improve frozen–thawed bull semen quality


Kaka, Asmatullah and Haron, Abd Wahid and Yusoff, Rosnina and Yimer, Nurhusien and Khumran, A. M. and Behan, Atique Ahmed and Ebrahimi, Mahdi (2015) Alpha-linolenic acid supplementation in Tris extender can improve frozen–thawed bull semen quality. Reproduction in Domestic Animals, 50 (1). pp. 29-33. ISSN 0936-6768; ESSN: 1439-0531


The study was conducted to evaluate the effects of α-linolenic acid (ALA) on frozen–thawed quality and fatty acid composition of bull sperm. For that, twenty-four ejaculates obtained from three bulls were diluted in a Tris extender containing 0 (control), 3, 5, 10 and 15 ng/ml of ALA. Extended semen was incubated at 37°C for 15 min, to allow absorption of ALA by sperm cell membrane. The sample was chilled for 2 h, packed into 0.25-ml straws and frozen in liquid nitrogen for 24 h. Subsequently, straws were thawed and evaluated for total sperm motility (computer-assisted semen analysis), membrane functional integrity (hypo-osmotic swelling test), viability (eosin-nigrosin), fatty acid composition (gas chromatography) and lipid peroxidation (thiobarbituric acid-reactive substances (TBARS)). A higher (p < 0.05) percentage of total sperm motility was observed in ALA groups 5 ng/ml (47.74 ± 07) and 10 ng/ml (44.90 ± 0.7) in comparison with control (34.53 ± 3.0), 3 ng/ml (34.40 ± 2.6) and 15 ng/ml (34.60 ± 2.9). Still, the 5 ng/ml ALA group presented a higher (p < 0.05) percentage of viable sperms (74.13 ± 0.8) and sperms with intact membrane (74.46 ± 09) than all other experimental groups. ALA concentration and lipid peroxidation in post-thawed sperm was higher in all treated groups when compared to the control group. As such, the addition of 5 ng/ml of ALA to Tris extender improved quality of frozen–thawed bull spermatozoa.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.1111/rda.12445
Publisher: Blackwell
Keywords: ALA; Tris extender; Bull sperm
Depositing User: Nabilah Mustapa
Date Deposited: 20 Jun 2015 09:28
Last Modified: 17 Sep 2015 07:09
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1111/rda.12445
URI: http://psasir.upm.edu.my/id/eprint/36366
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