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Acute toxicity study of zerumbone-loaded nanostructured lipid carrier on BALB/c mice model


Citation

Rahman, Heshu Sulaiman and Abdullah, Rasedee and Othman, Hemn Hassan and Chartrand, Max Stanley and Namvar, Farideh and Yeap, Swee Keong and Abdul Samad, Nozlena and Andas, Reena Joys and Muhammad Nadzri, Nabilah and Anasamy, Theebaa and Ng, Kuan Beng and How, Chee Wun (2014) Acute toxicity study of zerumbone-loaded nanostructured lipid carrier on BALB/c mice model. BioMed Research International, 2014. art. no. 563930. pp. 1-15. ISSN 2314-6133; ESSN: 2314-6141

Abstract

Zerumbone- (ZER-) loaded nanostructure lipid carrier (NLC) (ZER-NLC) prepared for its antileukemia effect in vitro was evaluated for its toxicological effects by observing changes in the liver, kidney, spleen, lung, heart, and brain tissues, serum biochemical parameters, total haemogram, and bone marrow stem cells. The acute toxicity study for ZER-NLC was conducted by orally treating BALB/c mice with a single dose with either water, olive oil, ZER, NLC, or ZER-NLC for 14 days. The animals were observed for clinical and behavioral abnormalities, toxicological symptoms, feed consumption, and gross appearance. The liver, kidney, heart, lung, spleen, and brain tissues were assessed histologically. Total haemogram was counted by hemocytometry and microhematocrit reader. Bone marrow examination in terms of cellular morphology was done by Wright staining with bone marrow smear. Furthermore, serum biochemical parameters were determined spectrophotometrically. Grossly all treated mice, their investigated tissues, serum biochemical parameters, total haemogram, and bone marrow were normal. At oral doses of 100 and 200 mg/kg ZER-NLC there was no sign of toxicity or mortality in BALB/c mice. This study suggests that the 50% lethal dose (LD50) of ZER-NLC is higher than 200 mg/kg, thus, safe by oral administration.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Institute of Bioscience
Institute of Tropical Forestry and Forest Products
DOI Number: https://doi.org/10.1155/2014/563930
Publisher: Hindawi Publishing Corporation
Keywords: Zerumbone- (ZER-) loaded nanostructure lipid carrier (NLC) (ZER-NLC); Acute toxicity; BALB/c mice
Depositing User: Nabilah Mustapa
Date Deposited: 04 Aug 2015 08:50
Last Modified: 29 Oct 2015 07:38
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2014/563930
URI: http://psasir.upm.edu.my/id/eprint/36326
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