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A rapid colorimetric screening method for vanillic acid and vanillin-producing bacterial strains


Zamzuri, Nur Ain and Abd. Aziz, Suraini and Abdul Rahim, Raha and Phang, Lai Yee and Mohamed Alitheen, Noorjahan Banu and Maeda, Toshinari (2014) A rapid colorimetric screening method for vanillic acid and vanillin-producing bacterial strains. Journal of Applied Microbiology, 116 (4). pp. 903-910. ISSN 1364-5072; ESSN: 1365-2672


Aim: To isolate a bacterial strain capable of biotransforming ferulic acid, a major component of lignin, into vanillin and vanillic acid by a rapid colorimetric screening method. Methods and Results: For the production of vanillin, a natural aroma compound, we attempted to isolate a potential strain using a simple screening method based on pH change resulting from the degradation of ferulic acid. The strain Pseudomonas sp. AZ10 UPM exhibited a significant result because of colour changes observed on the assay plate on day 1 with a high intensity of yellow colour. The biotransformation of ferulic acid into vanillic acid by the AZ10 strain provided the yield (Yp/s) and productivity (Pr) of 1·08 mg mg-1 and 53·1 mg L-1 h-1, respectively. In fact, new investigations regarding lignin degradation revealed that the strain was not able to produce vanillin and vanillic acid directly from lignin; however, partially digested lignin by mixed enzymatic treatment allowed the strain to produce 30·7 mg l-1 and 1·94 mg l-1 of vanillic acid and biovanillin, respectively. Conclusions: (i) The rapid colorimetric screening method allowed the isolation of a biovanillin producer using ferulic acid as the sole carbon source. (ii) Enzymatic treatment partially digested lignin, which could then be utilized by the strain to produce biovanillin and vanillic acid. Significance and Impact of the Study: To the best of our knowledge, this is the first study reporting the use of a rapid colorimetric screening method for bacterial strains producing vanillin and vanillic acid from ferulic acid.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1111/jam.12410
Publisher: The Society for Applied Microbiology
Keywords: Biotransformation; Biovanillin; Ferulic acid; Pseudomonas sp.; Rapid screening; Vanillic acid
Depositing User: Nabilah Mustapa
Date Deposited: 29 Dec 2015 12:50
Last Modified: 29 Dec 2015 12:50
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1111/jam.12410
URI: http://psasir.upm.edu.my/id/eprint/36244
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