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Validation of a HPLC method for determination of hydroxymethylfurfural in crude palm oil


Citation

Ariffin, Abdul Azis and Mohd Ghazali, Hasanah and Kavousi, Parviz (2014) Validation of a HPLC method for determination of hydroxymethylfurfural in crude palm oil. Food Chemistry, 154. pp. 102-107. ISSN 0308-8146; ESSN:1873-7072

Abstract / Synopsis

For the first time 5-hydroxymethyl-2-furaldehyde (HMF) was separated from crude palm oil (CPO), and its authenticity was determined using an RP-HPLC method. Separation was accomplished with isocratic elution of a mobile phase comprising water and methanol (92:8 v/v) on a Purospher Star RP-18e column (250 mm × 4.6 mm, 5.0 μm). The flow rate was adjusted to 1 ml/min and detection was performed at 284 nm. The method was validated, and results obtained exhibit a good recovery (95.58% to 98.39%). Assessment of precision showed that the relative standard deviations (RSD%) of retention times and peak areas of spiked samples were less than 0.59% and 2.66%, respectively. Further, the limit of detection (LOD) and LOQ were 0.02, 0.05 mg/kg, respectively, and the response was linear across the applied ranges. The crude palm oil samples analysed exhibited HMF content less than 2.27 mg/kg.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
DOI Number: https://doi.org/10.1016/j.foodchem.2013.12.082
Publisher: Elsevier
Keywords: 5-Hydroxymethylfurfural (HMF); Method validation; Precision; Recovery; Accuracy; Crude palm oil (CPO)
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 12 Feb 2016 02:37
Last Modified: 12 Feb 2016 02:37
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.foodchem.2013.12.082
URI: http://psasir.upm.edu.my/id/eprint/35957
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