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Toxicity and metabolism of layered double hydroxide intercalated with levodopa in a Parkinson's disease model


Kura, Aminu Umar and Mohd Ain, Nooraini and Hussein, Mohd Zobir and Fakurazi, Sharida and Hussein-Al-Ali, Samer Hasan (2014) Toxicity and metabolism of layered double hydroxide intercalated with levodopa in a Parkinson's disease model. International Journal of Molecular Sciences, 15 (4). pp. 5916-5927. ISSN 1422-0067


Layered hydroxide nanoparticles are generally biocompatible, and less toxic than most inorganic nanoparticles, making them an acceptable alternative drug delivery system. Due to growing concern over animal welfare and the expense of in vivo experiments both the public and the government are interested to find alternatives to animal testing. The toxicity potential of zinc aluminum layered hydroxide (ZAL) nanocomposite containing anti-Parkinsonian agent may be determined using a PC 12 cell model. ZAL nanocomposite demonstrated a decreased cytotoxic effect when compared to levodopa on PC12 cells with more than 80% cell viability at 100 µg/mL compared to less than 20% cell viability in a direct levodopa exposure. Neither levodopa-loaded nanocomposite nor the un-intercalated nanocomposite disturbed the cytoskeletal structure of the neurogenic cells at their IC50 concentration. Levodopa metabolite (HVA) released from the nanocomposite demonstrated the slow sustained and controlled release character of layered hydroxide nanoparticles unlike the burst uptake and release system shown with pure levodopa treatment.

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Official URL or Download Paper: http://www.mdpi.com/1422-0067/15/4/5916

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Institute of Advanced Technology
Institute of Bioscience
DOI Number: https://doi.org/10.3390/ijms15045916
Publisher: MDPI AG
Keywords: Zinc-aluminum; Nanocomposite; Cytotoxicity; PC 12; Levodopa; LDH
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 11 Feb 2016 04:38
Last Modified: 11 Feb 2016 04:38
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/ijms15045916
URI: http://psasir.upm.edu.my/id/eprint/35877
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