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Combination of superheated steam with laccase pretreatment together with size reduction to enhance enzymatic hydrolysis of oil palm biomass


Citation

Ahmad Rizal, Nur Fatin Athirah and Ibrahim, Mohamad Faizal and Zakaria @ Mamat, Mohd Rafein and Kamal Bahrin, Ezyana and Abd. Aziz, Suraini and Hassan, Mohd Ali (2018) Combination of superheated steam with laccase pretreatment together with size reduction to enhance enzymatic hydrolysis of oil palm biomass. Molecules, 23 (4). art. no. 811. pp. 1-14. ISSN 1420-3049

Abstract / Synopsis

The combination of superheated steam (SHS) with ligninolytic enzyme laccase pretreatment together with size reduction was conducted in order to enhance the enzymatic hydrolysis of oil palm biomass into glucose. The oil palm empty fruit bunch (OPEFB) and oil palm mesocarp fiber (OPMF) were pretreated with SHS and ground using a hammer mill to sizes of 2, 1, 0.5 and 0.25 mm before pretreatment using laccase to remove lignin. This study showed that reduction of size from raw to 0.25 mm plays important role in lignin degradation by laccase that removed 38.7% and 39.6% of the lignin from OPEFB and OPMF, respectively. The subsequent saccharification process of these pretreated OPEFB and OPMF generates glucose yields of 71.5% and 63.0%, which represent a 4.6 and 4.8-fold increase, respectively, as compared to untreated samples. This study showed that the combination of SHS with laccase pretreatment together with size reduction could enhance the glucose yield.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Tropical Forestry and Forest Products
DOI Number: https://doi.org/10.3390/molecules23040811
Publisher: MDPI
Keywords: Oil palm biomass; Physical pretreatment; Biological pretreatment; Lignin removal; Lignocellulosic biomass
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 13 Aug 2018 09:35
Last Modified: 13 Aug 2018 09:35
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules23040811
URI: http://psasir.upm.edu.my/id/eprint/35496
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