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Species identification of Malayan Gaur, Kedah-Kelantan and Bali cattle using polymerase chain reaction-restricted fragment length polymorphism


Mohd Nor, Siti Romaino and Zainal Abidin, Fazly Ann and Loo, Shu San and Mail, Mohd Hafiz and Darus, Mohamad Hafiz and Ismail, Mohd Iswadi and Kashiani, Pedram and Rosli, Muhammad Khairul Anwar and Syed Shabthar, Syed Mohamad Fahmi and Md Zain, Badrul Munir and Othman, Abas Mazni (2014) Species identification of Malayan Gaur, Kedah-Kelantan and Bali cattle using polymerase chain reaction-restricted fragment length polymorphism. Genetics and Molecular Research, 13 (1). pp. 406-414. ISSN 1676-5680


Mitochondrial DNA (mtDNA) is a useful genetic marker that can be used for species identification. The cytochrome b (Cyt b) gene is a suitable mtDNA candidate gene for use in phylogenetic analyses due to its sequence variability, which makes it appropriate for comparisons at the subspecies, species, and genus levels. This study was conducted to develop a rapid molecular method for species identification of Malayan gaur (Bos gaurus hubbacki), Kedah-Kelantan (KK) (Bos indicus), and Bali (Bos javanicus) cattle in Malaysia. DNA was extracted from blood samples of 8 Malayan gaurs, 30 KK, and 28 Bali cattle. A set of both specific and universal primers for the Cyt b gene were used in PCR amplification. DNA sequences obtained were then analyzed using BioEdit and Restriction Mapper softwares. The PCR products obtained from Cyt b gene amplification were then subjected to restriction enzyme digestion. The amplification, using both specific and universal primers, produced a 154- and a 603-bp fragment, respectively, in all three species. Two restriction enzymes, NlaIV and SspI, were used to obtain specific restriction profiles that allowed direct identification of Malayan gaur, KK, and Bali cattle. Our findings indicate that all three species can be identified separately using a combination of universal primers and the restriction enzyme SspI.

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Official URL or Download Paper: http://www.geneticsmr.com/articles/2777

Additional Metadata

Item Type: Article
Divisions: Institute of Tropical Agriculture
DOI Number: https://doi.org/10.4238/2014.January.21.8
Publisher: Fundacao de Pesquisas Cientificas de Ribeirao Preto
Keywords: Restriction enzymes; Malayan gaur; Kedah-Kelantan; Bali cattle; Seladang
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 11 Jan 2016 01:49
Last Modified: 11 Jan 2016 01:49
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.4238/2014.January.21.8
URI: http://psasir.upm.edu.my/id/eprint/35435
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