UPM Institutional Repository

Reduced neonatal regulatory T cell response to microbial stimuli associates with subsequent eczema in high-risk infants


Ismail , Intan Hakimah and Boyle, Robert J. and Mah, Li Jeen and Licciardi, Paul V. and Tang, Mimi L. K. (2014) Reduced neonatal regulatory T cell response to microbial stimuli associates with subsequent eczema in high-risk infants. Pediatric Allergy and Immunology, 25 (7). pp. 674-684. ISSN 0905-6157; ESSN: 1399-3038


Background Regulatory T cells (Treg) play an essential role in early immune programming and shaping the immune response towards a pro-allergic or tolerant state. We evaluated cord blood Treg and cytokine responses to microbial and non-microbial stimuli in infants at high risk of allergic disease and their associations with development of allergic disease in the first year. Methods Cord blood mononuclear cells from 72 neonates were cultured with toll-like receptors (TLR2) ligands: lipoteichoic acid (LTA) and heat-killed Lactobacillus rhamnosus GG (HKL); TLR4 ligand: lipopolysaccharide (LPS); ovalbumin (OVA); anti-CD3; or media for 48 h. Treg numbers and Treg cytokines were assessed in relation to allergic disease outcomes during the first year of life (eczema and atopic sensitization). Results Infants with eczema (n = 24) had reduced percentages of FoxP3hiCD25hi Treg in LTA (p = 0.01, adj p = 0.005) and HKL (p = 0.04, adj p = 0.02) stimulated cultures as well as reduced IL-10 (p = 0.01) production following HKL stimulation compared to those without eczema (n = 48). No differences in Treg or cytokine responses to LPS, OVA or anti-CD3 were seen. Infants who developed sensitization had lower percentages of Treg following TLR2 stimulation (but not other stimuli) compared to non-sensitized infants. Conclusions High-risk children who develop allergic disease in the first year of life have deficient Treg responses to microbial stimuli but not allergen from the time of birth, which may contribute to failure of immune tolerance development in infancy.

Download File

Full text not available from this repository.

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1111/pai.12303
Publisher: Wiley-Blackwell Publishing Ltd.
Keywords: Atopic sensitization; Cord blood; Cytokines; Eczema; Infants; Neonates; Microbial stimuli; Regulatory T cell; Toll-like receptor
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 31 Dec 2015 02:11
Last Modified: 31 Dec 2015 02:11
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1111/pai.12303
URI: http://psasir.upm.edu.my/id/eprint/35183
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item