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Proteomic analysis of the salt-responsive leaf and root proteins in the anticancer plant Andrographis paniculata Nees


Talei, Daryush and Valdiani, Alireza and Yusop, Mohd Rafii and Mahmood, Maziah (2014) Proteomic analysis of the salt-responsive leaf and root proteins in the anticancer plant Andrographis paniculata Nees. PLOS ONE, 9 (11). art. no. e112907. pp. 1-10. ISSN 1932-6203


Separation of proteins based on the physicochemical properties with different molecular weight and isoelectric points would be more accurate. In the current research, the 45-day-old seedlings were treated with 0 (control) and 12 dS m−1 of sodium chloride in the hydroponic system. After 15 days of salt exposure, the total protein of the fresh leaves and roots was extracted and analyzed using two-dimensional electrophoresis system (2-DE). The analysis led to the detection of 32 induced proteins (19 proteins in leaf and 13 proteins in the root) as well as 12 upregulated proteins (four proteins in leaf and eight proteins in the root) in the salt-treated plants. Of the 44 detected proteins, 12 were sequenced, and three of them matched with superoxide dismutase, ascorbate peroxidase and ribulose-1, 5-bisphosphate oxygenase whereas the rest remained unknown. The three known proteins associate with plants response to environmental stresses and could represent the general stress proteins in the present study too. In addition, the proteomic feedback of different accessions of A. paniculata to salt stress can potentially be used to breed salt-tolerant varieties of the herb.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
Institute of Tropical Agriculture
DOI Number: https://doi.org/10.1371/journal.pone.0112907
Publisher: Public Library of Science
Keywords: Proteomic analysis; Salt-responsive leaf; Root proteins; Anticancer plant; Andrographis paniculata Nees
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 30 Dec 2015 10:42
Last Modified: 01 Oct 2019 00:55
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1371/journal.pone.0112907
URI: http://psasir.upm.edu.my/id/eprint/35097
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