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Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells


Nami, Yousef and Abdullah, Norhafizah and Haghshenas, Babak and Awang Biak, Dayang Radiah and Rosli, Rozita and Khosroushahi, Ahmad Yari (2014) Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells. Anaerobe, 28. pp. 29-36. ISSN 1075-9964; ESSN: 1095-8274


Lactobacillus acidophilus is categorized as a probiotic strain because of its beneficial effects in human health and prevention of disease transmission. This study is aimed to characterize the probiotic potential of L. acidophilus 36YL originally isolated from the vagina of healthy and fertile Iranian women. The L. acidophilus 36YL strain was identified using 16S rDNA gene sequencing and characterized by biochemical methodologies, such as antibiotics susceptibility, antimicrobial activity, and acid and bile resistance. The bioactivity of the secretion of this strain on four human cancer cell lines (AGS, HeLa, MCF-7, and HT-29) and one normal cell line (HUVEC) was evaluated by cytotoxicity assay and apoptosis analysis. This newly isolated strain was found to exhibit notable probiotic properties, such as admirable antibiotic susceptibility, good antimicrobial activity, and favorable resistance to acid and bile salt. The results of bioactivity assessment demonstrated acceptable anticancer effects on the four tested cancer cell lines and negligible side effects on the assayed normal cell line. Our findings revealed that the anticancer effect of L. acidophilus 36YL strain secretions depends on the induction of apoptosis in cancer cells. L. acidophilus 36YL strain is considered as a nutraceutical alternative or a topical medication with a potential therapeutic index because of the absence of cytotoxicity to normal cells, but effective toxicity to cancer cell lines.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
Institute of Bioscience
DOI Number: https://doi.org/10.1016/j.anaerobe.2014.04.012
Publisher: Elsevier
Keywords: Apoptosis; Anticancer; Anti-microbe; Antibiotic susceptibility; Cytotoxicity
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 29 Dec 2015 07:51
Last Modified: 29 Dec 2015 07:51
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.anaerobe.2014.04.012
URI: http://psasir.upm.edu.my/id/eprint/35065
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