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Prioritization of natural extracts by LC-MS-PCA for the identification of new photosensitizers for photodynamic therapy


Samat, Norazwana and Tan, Pei Jean and Shaari, Khozirah and Abas, Faridah and Lee, Hong Boon (2014) Prioritization of natural extracts by LC-MS-PCA for the identification of new photosensitizers for photodynamic therapy. Analytical Chemistry, 86 (3). pp. 1324-1331. ISSN 0003-2700; ESSN: 1520-6882


Photodynamic therapy (PDT) is an alternative treatment for cancer that involves administration of a photosensitive drug or photosensitizer that localizes at the tumor tissue followed by in situ excitation at an appropriate wavelength of light. Tumour tissues are then killed by cytotoxic reactive oxygen species generated by the photosensitizer. Targeted excitation and photokilling of affected tissues is achieved through focal light irradiation, thereby minimizing systemic side effects to the normal healthy tissues. Currently, there are only a small number of photosensitizers that are in the clinic and many of these share the same structural core based on cyclic tetrapyrroles. This paper describes how metabolic tools are utilized to prioritize natural extracts to search for structurally new photosensitizers from Malaysian biodiversity. As proof of concept, we analyzed 278 photocytotoxic extracts using a hyphenated technique of liquid chromatography–mass spectrometry coupled with principal component analysis (LC–MS-PCA) and prioritized 27 extracts that potentially contained new photosensitizers for chemical dereplication using an in-house UPLC-PDA-MS-Photocytotoxic assay platform. This led to the identification of 2 new photosensitizers with cyclic tetrapyrrolic structures, thereby demonstrating the feasibility of the metabolic approach.

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Official URL or Download Paper: http://pubs.acs.org/doi/abs/10.1021/ac403709a

Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Institute of Bioscience
DOI Number: https://doi.org/10.1021/ac403709a
Publisher: American Chemical Society
Keywords: Natural extracts; Tumour tissues; Photosensitizers; Photodynamic therapy
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 29 Dec 2015 07:40
Last Modified: 27 Jan 2016 03:35
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1021/ac403709a
URI: http://psasir.upm.edu.my/id/eprint/35056
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