Simple Search:

Polymerase chain reaction assay targeting cytochrome b gene for the detection of dog meat adulteration in meatball formulation


Citation

Rahman, Md. Mahfujur and Ali, Md. Eaqub and Abd Hamid, Sharifah Bee and Mustafa, Shuhaimi and Hashim, Uda and Hanapi, Ummi Kalthum (2014) Polymerase chain reaction assay targeting cytochrome b gene for the detection of dog meat adulteration in meatball formulation. Meat Science, 97 (4). pp. 404-409. ISSN 0309-1740; ESSN: 1873-4138

Abstract / Synopsis

A polymerase chain reaction (PCR) assay for the assessment of dog meat adulteration in meatballs was developed. The assay selectively amplified a 100-bp region of canine mitochondrial cytochrome b gene from pure, raw, processed and mixed backgrounds. The specificity of the assay was tested against 11 animals and 3 plants species, commonly available for meatball formulation. The stability of the assay was proven under extensively autoclaving conditions that breakdown target DNA. A blind test from ready to eat chicken and beef meatballs showed that the assay can repeatedly detect 0.2% canine meat tissues under complex matrices using 0.04 ng of dog DNA extracted from differentially treated meatballs. The simplicity, stability and sensitivity of the assay suggested that it could be used in halal food industry for the authentication of canine derivatives in processed foods.


Download File

Full text not available from this repository.

Additional Metadata

Item Type: Article
Divisions: Halal Products Research Institute
DOI Number: https://doi.org/10.1016/j.meatsci.2014.03.011
Publisher: Elsevier
Keywords: Canine derivatives; Meatballs; Extensive autoclaving; Halal food industry
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 24 Dec 2015 13:46
Last Modified: 24 Dec 2015 13:46
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.meatsci.2014.03.011
URI: http://psasir.upm.edu.my/id/eprint/34954
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item