UPM Institutional Repository

Pathogenesis of systemic Candida glabrata infection in an intravenous challenge murine model


Chin, Voon Kin and Foong, Kuan Jeang and Abdullah, Maha and Basir, Rusliza and Mohtarrudin, Norhafizah and Chong, Pei Pei (2014) Pathogenesis of systemic Candida glabrata infection in an intravenous challenge murine model. Journal of Pure and Applied Microbiology, 8 (5). pp. 3421-3428. ISSN 0973-7510


The incidence of systemic infection caused by C. glabrata is increasing in immunocompromised patients and resulted in high mortality rate due to antifungal resistance. The pathogenesis underlying C. glabrata infection still remains elusive and requires extensive study on it. Hence, this study was aimed to elucidate the pathogenesis of a clinical C. glabrata isolate from a Malaysian patient in an intravenous challenged murine model. Mice were challenged intravenously with C. glabrata (1x10^8 organisms/mouse) via lateral tail vein and parameters such as quantitative yeast culture, red blood cells and haemoglobin counts, blood plate assay and histopathology were adopted to evaluate the pathogenesis of systemic C. glabrata infection. Transcript level of erythropoietin from blood at day 7 post infection was quantified via RT-qPCR. Kidneys of infected mice have highest fungal recovery rate as compared to other organs and there were yeast infiltration with mild inflammation seen in kidney and brain tissues. Red blood cells and haemoglobin counts were reduced throughout the infection period and this reduction which might be associated with the action of haemolysin enzyme of C. glabrata in conjunction with iron scavenging for the fungal growth. Erythropoietin mRNA level was found to be up-regulated in blood which indicated a possible role for erythropoietin in compensating the red blood cells loss throughout the infection period. This study reflected the core events during systemic C. glabrata infection and involvement of erythropoietin which could be of clinical relevance during systemic C. glabrata infection. However, further comprehensive in vitro and in vivo studies are warranted.

Download File

PDF (Abstract)
Pathogenesis of systemic Candida glabrata infection in an intravenous challenge murine model.pdf

Download (36kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Publisher: Oriental Scientific Publishing Co.
Keywords: C. glabrata; Erythropoietin; Haemolysin; Red blood cells; Haemoglobin counts; Histopathology; Quantitative yeast counts
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 23 Dec 2015 04:01
Last Modified: 05 Sep 2016 09:58
URI: http://psasir.upm.edu.my/id/eprint/34836
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item