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Optimization of γ-aminobutyric acid production by Lactobacillus plantarum Taj-Apis362 from honeybees


Tajabadi, Naser and Ebrahimpour, Afshin and Baradaran, Ali and Abdul Rahim, Raha and Mahyudin, Nor Ainy and Abdul Manap, Mohd Yazid and Abu Bakar, Fatimah and Saari, Nazamid (2015) Optimization of γ-aminobutyric acid production by Lactobacillus plantarum Taj-Apis362 from honeybees. Molecules, 20 (4). pp. 6654-6669. ISSN 1420-3049


Dominant strains of lactic acid bacteria (LAB) isolated from honey bees were evaluated for their γ-aminobutyric acid (GABA)-producing ability. Out of 24 strains, strain Taj-Apis362 showed the highest GABA-producing ability (1.76 mM) in MRS broth containing 50 mM initial glutamic acid cultured for 60 h. Effects of fermentation parameters, including initial glutamic acid level, culture temperature, initial pH and incubation time on GABA production were investigated via a single parameter optimization strategy. The optimal fermentation condition for GABA production was modeled using response surface methodology (RSM). The results showed that the culture temperature was the most significant factor for GABA production. The optimum conditions for maximum GABA production by Lactobacillus plantarum Taj-Apis362 were an initial glutamic acid concentration of 497.97 mM, culture temperature of 36 °C, initial pH of 5.31 and incubation time of 60 h, which produced 7.15 mM of GABA. The value is comparable with the predicted value of 7.21 mM.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Food Science and Technology
DOI Number: https://doi.org/10.3390/molecules20046654
Publisher: MDPI
Keywords: γ-aminobutyric acid; Response surface methodology; Glutamic acid; Lactobacillus plantarum Taj-Apis362
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 22 Dec 2015 09:04
Last Modified: 22 Dec 2015 09:04
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules20046654
URI: http://psasir.upm.edu.my/id/eprint/34765
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