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Novel SNPs in heat shock protein 70 gene and their association with sperm quality traits of Boer goats and Boer crosses


Nikbin, Saeid and Panandam, Jothi Malar and Yaakub, Halimatun and Marimuthu, Murugaiyah and Sazili, Awis Qurni (2014) Novel SNPs in heat shock protein 70 gene and their association with sperm quality traits of Boer goats and Boer crosses. Animal Reproduction Science, 146 (3-4). pp. 176-181. ISSN 0378-4320; ESSN: 1873-2232


The semen quality of bucks affects the reproduction performance of the herd and is influenced by genetic and non-genetic factors. Heat shock protein 70 (HSP70) is considered as an important gene affecting semen quality traits. The objectives of this study are to find single nucleotide polymorphisms in HSP70 coding region and their association with semen quality traits on Boer and Boer cross bucks. DNA isolated from 53 goats (36 pure South African Boer and 17 Boer crosses) was subjected to PCR amplification of the exon 1 region of the caprine HSP70 gene. Single-strand conformation polymorphism (SSCP) was used to detect polymorphisms and the variant DNA fragments were sequenced. Two synonymous SNPs (74A > C (ss836187517) and 191C > G (ss836187518)) were detected. Qualities of fresh and post-thaw semen were evaluated for sperm concentration, semen volume, sperm motility and velocity traits, live sperm percentage, and abnormal sperm rate. The C allele of ss836187517 and G allele of ss836187518 were at higher frequencies in both the breeds. The C allele of ss836187517 appeared to be the favorable allele for semen concentration, progressive motility of fresh semen, and motility and sperm lateral head displacement of post-thaw semen. A negative overdominance was observed for ss836187517 alleles on velocity traits of post-thaw semen. The C allele of ss836187518 was favorable for sperm concentration and progressive motility. Results herein suggest that the SNPs in HSP70 may affect on semen quality in tropical regions and specially on the potential of semen for freezing.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.1016/j.anireprosci.2014.03.001
Publisher: Elsevier
Keywords: Male reproduction; Single nucleotide polymorphisms; Single-strand conformation polymorphism; Fresh semen quality; Post-thaw semen quality
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 18 Dec 2015 01:33
Last Modified: 18 Dec 2015 01:33
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.anireprosci.2014.03.001
URI: http://psasir.upm.edu.my/id/eprint/34676
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