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Molecular cloning of a putative Acanthus ebracteatus- 9-cis-epoxycarotenoid deoxygenase (AeNCED) and its overexpression in rice


Citation

Sultana, Shahanaz and Turečková, Veronika and Ho, Chai Ling and Napis, Suhaimi and Namasivayam, Parameswari (2014) Molecular cloning of a putative Acanthus ebracteatus- 9-cis-epoxycarotenoid deoxygenase (AeNCED) and its overexpression in rice. Journal of Crop Science and Biotechnology, 17 (4). pp. 239-246. ISSN 1975-9479; ESSN: 2005-8276

Abstract

9-Cis-epoxycarotenoid dioxygenase (NCED) is an important enzyme which catalyzes the oxidative cleavage of abscisic acid (ABA), a plant growth regulator which plays a vital role in stress tolerance. In this study, a cDNA sequence encoding NCED from a mangrove plant (Acanthus ebracteatus) was analyzed and overexpressed in rice. Analysis of the deduced amino acid sequence of AeNCED revealed an open reading frame of 1,638 bp encoding a protein of 545 amino acids, with a 123 bp 5’-untranslated region (UTR) and a 259 bp 3’-UTR. The deduced amino acid sequence of AeNCED is more than 80% identical to the amino acid sequences of carotenoid cleavage dioxygenase from carrot, tomato, and coffee. The RNA encoding AeNCED was detected in transgenic rice (Oryza sativa cv. BRRI dhan29) plants overexpressing this cDNA. These plants only showed significantly higher tolerance to salinity at germination and better performance at seedling stages. The levels of ABA in transgenic rice seedlings overexpressing AeNCED treated with 100 mM NaCl for 24 hours were higher than those of untransformed plants. However, a higher level of dihydrophaseic acid (DPA) and ABA glucose ester (ABA-GE) were also observed in these transgenic plants suggesting that rapid degradation of ABA through a self-regulation mechanism.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1007/s12892-014-0006-4
Publisher: Korean Society of Crop Science
Keywords: 9-cis-epoxycarotenoid dioxygenase; Overexpression; Rice; Salt tolerance; Sequence analysis
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 16 Dec 2015 02:00
Last Modified: 22 Jan 2016 03:12
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s12892-014-0006-4
URI: http://psasir.upm.edu.my/id/eprint/34576
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